Three bacterial strains efficiently reduce selenite to selenium nanoparticles in cell membranes

文献类型： 外文期刊

第一作者： Li, Ruixia

作者： Li, Ruixia;Chen, Wenqiang;Huang, Siyuan;Jiang, Daihua;Huang, Xuejiao;Zhu, Zhengjie;Li, Chong

作者机构：

关键词： Antioxidant; Bio-nano selenium; Cell membrane; Selenite reduction

期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.8 ）

ISSN： 1471-2180

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Microbial synthesis of selenium nanoparticles (SeNPs) as a fertilizer can promote the development of selenium-rich agricultural products. However, most known selenium-reduction strains exhibit a tolerance to selenite of <= 100 mmol/L and possess relatively low reduction efficiency. In this study, three strains capable of tolerating selenite concentrations of > 300 mmol/L were screened from selenium-rich soil in Bama, Guangxi, China. Based on 16 S rRNA gene sequence analysis, the three strains were identified as Citrobacter sp.BM-1, Providencia sp.BM-2, and Brucella sp.BM-3. Notably, Brucella sp.BM-3 represents a novel selenium-reducing bacteria. All three strains reduced SeO32- to SeNPs on the cell membrane and subsequently released these nanoparticles outside the cells, forming spherical SeNPs with a particle size of 210-221 nm. Furthermore, qPCR analysis revealed that the selenium-reduction mechanisms in these strains primarily involve the glutathione pathway, which is catalyzed by nitrate reductase and sulfate reductase. These findings suggest that Citrobacter sp.BM-1, Providencia sp.BM-2, and Brucella sp.BM-3 are promising candidates for the synthesis of SeNPs.

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