Characterization and Comparative Expression Profiling of Browning Response in Medinilla formosana after Cutting

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Wang, Yiting;Song, Xijiao;Chen, Jianping;Liz, Kunfeng

作者机构：

关键词： transcriptome;high-throughput sequencing;browning;tissue culture;Medinilla;cutting;gene expression profiling

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Plant browning is a recalcitrant problem for in vitro culture and often leads to poor growth of explants and even failure of tissue culture. However, the molecular mechanisms underlying browning-induced physiological processes remain unclear. Medinilla is considered one of the most difficult genera for tissue culture owning to its severe browning. In the present study, intact aseptic plantlets of Meelinilla formosana Hayata previously obtained by ovary culture, were used to explore the characteristics and molecular mechanism of the browning response. Successive morphological and anatomical observations after cutting showed that the browning of M. formosana was not lethal but adaptive. De novo transcriptome and digital gene expression (DGE) profiling using Illumina high-throughput sequencing were then used to explore molecular regulation after cutting. About 7.5 million tags of de novo transcriptome were obtained and 58,073 unigenes were assembled and annotated. A total of 6,431 differentially expressed genes (DEGs) at three stages after cutting were identified, and the expression patterns of these browning-related genes were clustered and analyzed. A number of putative DEGs involved in signal transduction and secondary metabolism were particularly studied and the potential roles of these cutting-responsive mRNAs in plant defense to diverse abiotic stresses are discussed. The DGE profiling data were also validated by quantitative RT-PCR analysis. The data obtained in this study provide an excellent resource for unraveling the molecular mechanisms of browning processes during in vitro tissue culture, and lay a foundation for future studies to inhibit and eliminate browning damage.

分类号：

  • 相关文献

[1]An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro. Wang, Y.,Feng, D. D.,Chen, J. P.,Li, X. B.. 2015

[2]Comparative Transcriptomic Analyses of Vegetable and Grain Pea (Pisum sativum L.) Seed Development. Liu, Na,Zhang, Guwen,Xu, Shengchun,Hu, Qizan,Gong, Yaming,Mao, Weihua. 2015

[3]Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection. Zhang, Xiang,Chen, Songlin,Chen, Yadong,Shao, Changwei,Wang, Qilong,Lu, Yang,Gong, Guangye,Sha, Zhenxia,Zhang, Xiang,Ding, Shaoxiong,Wang, Shaolin,Liu, Yang.

[4]RNA-seq for comparative transcript profiling of kenaf under salinity stress. Li, Hui,Li, Defang,Chen, Anguo,Tang, Huijuan,Li, Jianjun,Huang, Siqi.

[5]Transcript profiling analysis reveals crucial genes regulating main metabolism during adventitious root formation in cuttings of Morus alba L.. Du, XiaoLong,Zhang, XiaoFeng,Nie, Hao,Liu, MingLu,Cheng, Jia Ling,Cheng, Jia Ling.

[6]Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen. Yan, Shao Peng,Wang, Fang,Sun, Li Na,Song, Xing Shun,Yang, Rui Hua,Song, Xing Shun. 2017

[7]Comprehensive Analysis of R2R3-MYB Genes During Adventitious Root Formation in Cuttings of Morus alba. Du, Xiao Long,Cao, Xu,Yin, Chao Rui,Tang, Zhuang,Ban, Yue Yuan,Cheng, Jia Ling,Du, Wei.

[8]Effects of Cutting on Alfalfa Yield and Quality in Northeast China. Chen Ji-Shan,Gao Cao,Di Gui-Li,Zhu Rui-Fen,Zhang Yue-Xue.

[9]Comparative transcriptome analysis of basal and zygote-located tip regions of peanut ovaries provides insight into the mechanism of light regulation in peanut embryo and pod development. Zhang, Ye,Wang, Pengfei,Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Zhao, Shuzhen,Wang, Xingjun,Zhang, Ye,Gao, Chao,Wang, Xingjun. 2016

[10]Gene expression profiling related to powdery mildew resistance in wheat with the method of suppression subtractive hybridization. Luo, M,Kong, XY,Huo, NX,Zhou, RH,Jia, JZ. 2002

[11]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

[12]Molecular identification, polymorphism, and expression analysis of major histocompatibility complex class IIA and B genes of turbot (Scophthalmus maximus). Zhang, Yu-Xi,Chen, Song-Lin.

[13]Gene expression profiling reveals U1 snRNA regulates cancer gene expression. Cheng, Zhi,Sun, Yu,Niu, Xiaoran,Shang, Yingchun,Gao, Shan,Zhang, Tao,Cheng, Zhi,Sun, Yu,Niu, Xiaoran,Shang, Yingchun,Gao, Shan,Zhang, Tao,Sun, Yu,Gao, Shan,Ruan, Jishou,Cheng, Zhi,Chen, Ze,Cheng, Zhi,Chen, Ze. 2017

[14]Quantitative trait loci identification, fine mapping and gene expression profiling for ovicidal response to whitebacked planthopper (Sogatella furcifera Horvath) in rice (Oryza sativa L.). Yang, Yaolong,Xu, Jie,Leng, Yujia,Hu, Jiang,Huang, Lichao,Wang, Lan,Guo, Longbiao,Qian, Qian,Zeng, Dali,Xiong, Guosheng,Li, Jiayang,Zeng, Dali,Chen, Feng. 2014

[15]Screening and analyzing genes associated with Amur tiger placental development. Li, Q.,Ma, J. Z.,Zhang, M. H.,Lu, T. F.,Hu, P. F.,Sun, B.,Wang, W. J.,Wang, K. F.,Zhang, W. X.,Guan, W. J.,Ma, Y. H.,Liu, D.,Chen, J.. 2014

[16]Transcriptome analysis of peach [Prunus persica (L.) Batsch] stigma in response to low-temperature stress with digital gene expression profiling. Jiao, Yun,Jiao, Yun,Shen, Zhijun,Yan, Juan.

[17]Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress. Wang, Xiaoli,Wang, Xiaoli,Li, Xiaodong,Zhang, Sheng,Korpelainen, Helena,Li, Chunyang.

[18]Genome-wide profiling of changes in gene expression in response to infection of the japonica rice variety Yunyin by Magnaporthe oryzae. Huang, Feng,Lian, Ling,He, Wei,Zhu, Yongsheng,Cai, Qiuhua,Xie, Huaan,Zhang, Jianfu,Huang, Feng,Lian, Ling,He, Wei,Zhu, Yongsheng,Cai, Qiuhua,Xie, Huaan,Zhang, Jianfu,Huang, Feng,Lian, Ling,He, Wei,Zhu, Yongsheng,Cai, Qiuhua,Xie, Huaan,Zhang, Jianfu,Huang, Feng,Lian, Ling,He, Wei,Zhu, Yongsheng,Cai, Qiuhua,Xie, Huaan,Zhang, Jianfu,Huang, Feng.

[19]Two Endogenous Substrates for Polyphenoloxidase in Pericarp Tissues of Postharvest Rambutan Fruit. Su, Weiqiang,Peng, Hongxiang,Zhu, Jianhua,Xu, Liangxiong,Marti Bruna, Nuria. 2010

[20]Variations in contents of (-)-epicatechin and activities of phenylalanine ammonialyase and polyphenol oxidase of longan fruit during development. Yu, Chunyan,Sun, Jian,Yang, Bao,Jiang, Yueming,Xiang, Xu. 2010

作者其他论文 更多>>

微信小程序