Secretome analysis of rice suspension-cultured cells infected by Xanthomonas oryzae pv. oryza (Xoo)

文献类型： 外文期刊

第一作者： Chen, Xian

作者： Chen, Xian;Chen, Xian;Deng, Zhiping;Yu, Chulang;Yan, Chengqi;Chen, Jianping

作者机构：

关键词： Bacterial blight;2-D DIGE;MS;Secretome;Xoo3654

期刊名称：PROTEOME SCIENCE （ 影响因子：2.48； 五年影响因子：2.619 ）

ISSN： 1477-5956

年卷期： 2016 年 14 卷

页码：

收录情况： SCI

摘要： Background: Rice bacterial blight (BB) caused by Xanthomonas oryzae pv.oryzae (Xoo) is one of the most devastating bacterial diseases in rice-growing regions worldwide. The rice-Xoo interaction is a classical model for studying the interaction between plants and pathogens. Secreted proteins play important roles in plant-bacterial interactions, but are poorly studied in the rice-Xoo system. Rice cv. Nipponbare is highly susceptible to Xoo. Here, we used two-dimensional difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF mass spectrometry (MS), to investigate secreted proteins in Nipponbare embryo cell suspension culture infected by Xoo. Results: A total of 32 protein spots changed significantly (p<0.05) by more than 1.5 fold in gel intensity after Xoo inoculation, and were identified by MS. They represent protein products of 11 unique genes, seven from rice and four from Xoo. Of the rice proteins, six up-regulated proteins are involved in cell wall modification, the TCA cycle, glycolysis and redox, while a down-regulated protein, CHIT16, is involved in plant defense. Quantitative Real-Time PCR showed that transcript levels were not correlated with secreted protein levels. Of the Xoo proteins, three of them were possibly located in the extracellular space as shown by transient expression assays in rice protoplasts. Two of the Xoo proteins were previously reported to be likely involved in pathogenicity, and the third gene, Xoo3654, is likely a negative regulator of Xoo virulence as its overexpression reduced Xoo pathogenicity in our study. Conclusion: Among the secreted proteins that responded to Xoo inoculation, we identified rice proteins involved in cell defense and Xoo proteins involved in pathogenicity. Our study also showed that Xoo3654 (X2) protein is likely a novel negative regulator of Xoo virulence. These results not only help us better understand the interaction between susceptible rice and Xoo, but also serve as a reference for studying the interaction between other plants and their pathogens.

分类号：

  • 相关文献

[1]Differential expression and modification of proteins during ontogenesis in Malus domestica. Cao, Xin,Gao, Yan,Wang, Yi,Han, Zhen H.,Zhang, Xin Z.,Li, Chun M.,Zhao, Yong B.. 2011

[2]Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. Li, Yuming,Wu, Zhuanchang,Liu, Ke,Qi, Pengfei,Xu, Jinpeng,Wei, Jianchao,Li, Beibei,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong.

[3]Insight into Enzymatic Degradation of Corn, Wheat, and Soybean Cell Wall Cellulose Using Quantitative Secretome Analysis of Aspergillus fumigatus. Ghimire, Prakriti Sharma,Ouyang, Haomiao,Yang, Jinghua,Lu, Yang,Jin, Cheng,Ghimire, Prakriti Sharma,Jin, Cheng,Wang, Qian,Luo, Yuanming,Shi, Bo,Ghimire, Prakriti Sharma.

[4]Effects of Different Substrates on Lignocellulosic Enzyme Expression, Enzyme Activity, Substrate Utilization and Biological Efficiency of Pleurotus Eryngii. Xie, Chunliang,Yan, Li,Gong, Wenbing,Zhu, Zuohua,Tan, Senwei,Chen, Du,Hu, Zhenxiu,Peng, Yuande. 2016

[5]Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation. Xie, Chunliang,Luo, Wei,Li, Zhimin,Yan, Li,Zhu, Zuohua,Wang, Jing,Hu, Zhenxiu,Peng, Yuande.

[6]Characterization of the secretome of Plasmopara viticola by de novo transcriptome analysis. Li, Xinlong,Xiang, Jiang,Zhang, Yali,Lu, Jiang,Yin, Ling,Dry, Ian B.,Qu, Junjie.

[7]Antioxidant and tyrosinase inhibitory activity of Rosa roxburghii fruit and identification of main bioactive phytochemicals by UPLC-Triple-TOF/MS. Zeng, Fangfang,Limwachiranon, Jarukitt,Li, Li,Feng, Simin,Luo, Zisheng,Ge, Zhiwei,Wang, Yansheng.

[8]Synthesis and spectral analysis of 12H-dibenzo[1,3,2]dioxaphosphocins. Chen, WY,Li, CJ. 2003

[9]Marker-assisted breeding of Indonesia local rice variety Siputeh for semi-dwarf phonetype, good grain quality and disease resistance to bacterial blight. Luo, Yanchang,Yin, Zhongchao,Zakaria, Sabaruddin,Basyah, Bakhtiar,Luo, Yanchang,Ma, Tingchen,Li, Zefu,Yang, Jianbo,Yin, Zhongchao. 2014

[10]Assessment of antibacterial properties and the active ingredient of plant extracts and its effect on the performance of crucian carp (Carassius auratus gibelio var. E'erqisi, Bloch). Lu, Chunxia,Luo, Xiaoling,Luo, Ruifeng,Chen, Xia,Xing, Lijie,Tang, Zonggui,Li, Hongmin. 2013

[11]Detecting major QTL associated with resistance to bacterial blight using a set of rice reciprocal introgression lines with high density SNP markers. Zhang, Fan,Xu, Meirong,Wang, Wensheng,Xu, Jianlong,Zhou, Yongli,Li, Zhikang,Xie, Xuewen,Li, Zhikang,Xu, Jianlong,Zhou, Yongli.

[12]RFLP mapping and race specificity of bacterial blight resistance genes (QTLs) in rice. Luo, LJ,Mei, HW,Zhao, XH,Zhong, DB,Wang, YP,Yu, XQ,Ying, CS,Li, ZK,Paterson, AH,Wang, DL,Tabien, RE,Zhu, L,Stansel, JW.

[13]Identification of Quantitative Trait Loci for Bacterial Blight Resistance Derived from Oryza meyeriana and Agronomic Traits in Recombinant Inbred Lines of Oryza sativa. Chen, Li-Na,Yang, Yong,Yan, Cheng-Qi,Wang, Ming,Yu, Chu-Lang,Zhou, Jie,Cheng, Ye,Cheng, Xiao-Yue,Chen, Jian-Ping,Chen, Li-Na,Zhang, Wei-Lin,Cheng, Xiao-Yue. 2012

[14]Transgenic rice plants overexpressing BBTI4 confer partial but broad-spectrum bacterial blight resistance. Hu, Songnian,Pang, Zhiqian,Zhou, Zhuangzhi,Yin, Dedong,Lv, Qiming,Wang, Lixiang,Wang, Jing,Li, Xiaobing,Zhao, Xianfeng,Jiang, Guanghuai,Zhu, Lihuang,Pang, Zhiqian,Lv, Qiming,Xu, Xiao,Lan, Jinping,Liu, Guozhen. 2013

[15]Genetic Overlap in the Quantitative Resistance of Rice at the Seedling and Adult Stages to Xanthomonas oryzae pv. oryzae. Zhou, Yong-Li,Xie, Xue-Wen,Xu, Mei-Rong,Zang, Jin-Ping,Zhu, Ling-Hua,Xu, Jian-Long,Li, Zhi-Kang,Li, Zhi-Kang.

[16]Concentration and dissipation of chlorantraniliprole and thiamethoxam residues in maize straw, maize, and soil. Zheng, Yongquan,He, Min,Jia, Hong C.,Song, Dan.

[17]Quantification of near-attomole gibberellins in floral organs dissected from a single Arabidopsis thaliana flower. Li, Dongmei,Guo, Zhenpeng,Liu, Cuimei,Chen, Yi,Li, Dongmei,Guo, Zhenpeng,Liu, Cuimei,Chen, Yi,Li, Jincheng,Xu, Wenzhong,Chen, Yi.

[18]FLAVONOIDS ACCUMULATE IN CELL WALLS, MIDDLE LAMELLAE AND CALLOSE-RICH PAPILLAE DURING AN INCOMPATIBLE INTERACTION BETWEEN XANTHOMONAS CAMPESTRIS PV MALVACEARUM AND COTTON. Dai, GH,Nicole, M,Andary, C,Martinez, C,Bresson, E,Boher, B,Daniel, JF,Geiger, JP.

[19]Identification and expression analysis of components involved in rice Xa21-mediated disease resistance signalling. Bai, H.,Gan, Q.,Zhu, L. H.,Bai, H.,Bai, H.,Cao, Y. H.,Wu, L.,Lan, J. P.,Wang, X. Y.,Hou, M. M.,Li, L. Y.,Liu, L. J.,Liu, G. Z.,Hao, Y. J.,Yin, C. C..

[20]Determination of beta(2)-Agonists in Porcine Urine by Molecularly Imprinted Solid Phase Extraction Followed Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Detection. Wang, Peilong,Li, Yang,Wang, Ruiguo,Zhang, Wei,Cheng, Jie,Wang, Jing,Su, Xiaoou,Ye, Zhihua,Wang, Peilong,Li, Yang,Wang, Ruiguo,Zhang, Wei,Cheng, Jie,Wang, Jing,Su, Xiaoou,Ye, Zhihua. 2013

作者其他论文 更多>>

微信小程序