P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts

文献类型： 外文期刊

第一作者： Liu, Xiao-Ya

作者： Liu, Xiao-Ya;Liu, Xiao-Ya;Yang, Jian;Xie, Li;Li, Jing;Song, Xi-Jiao;Chen, Jian-Ping;Zhang, Heng-Mu

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2015 年 160 卷 5 期

页码：

收录情况： SCI

摘要： The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序