High-affinity truncated aptamers for detection of Cronobacter spp with magnetic separation-assisted DNAzyme-driven 3D DNA walker

文献类型： 外文期刊

第一作者： Yang, Ningru

作者： Yang, Ningru;Ding, Ning;Qi, Shuo;Shang, Zixuan;Ma, Pengfei;Khan, Imran Mahmood;Wang, Zhouping;Xia, Yu;Khan, Imran Mahmood;Wang, Zhouping;Zhang, Yin;Zhang, Lili;Wang, Zhouping

作者机构：

关键词： Cronobacter spp.; Aptamer; Truncation; DNA walker; Au nanoparticles; Fluorescence detection

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.7； 五年影响因子：5.4 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 3 期

页码：

收录情况： SCI

摘要： After optimizing the original aptamer sequence by truncation strategy, a magnetic separation-assisted DNAzyme-driven 3D DNA walker fluorescent aptasensor was developed for detecting the food-borne pathogen Cronobacter species. Iron oxide magnetic nanoparticles (MNPs) modified with a hybrid of truncated aptamer probe and DNAzyme strand (AP-E1) denoted as MNPs@AP-E1, were employed as capture probes. Simultaneously, a DNAzyme-driven 3D-DNA walker was utilized as the signal amplification element. The substrate strand (Sub) was conjugated with the gold nanoparticles (AuNPs), resulting in the formation of AuNPs@Sub, which served as a 3D walking track. In the presence of the target bacteria and Mg2+, E1-DNAzyme was activated and moved along AuNPs@Sub, continuously releasing the signal probe. Under optimized conditions, a strong linear correlation was observed for Cronobacter sakazakii (C. sakazakii) in the concentration range 10(1) to 10(6) CFU mL(-1), with a low detection limit of 2 CFU mL(-1). The fluorescence signal responses for different Cronobacter species exhibited insignificant differences, with a relative standard deviation of 3.6%. Moreover, the aptasensor was successfully applied to determine C. sakazakii in real samples with recoveries of 92.86%-108.33%. Therefore, the novel method could be a good candidate for ultra-sensitive and selective detection of Cronobacter species without complex manipulation.

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