Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

文献类型: 外文期刊

第一作者: Han, Xueying

作者: Han, Xueying;Chen, Jianping;Han, Xueying;Yang, Yong;Wang, Xuming;Zhou, Jie;Yu, Chulang;Cheng, Chen;Cheng, Ye;Yan, Chengqi;Chen, Jianping;Zhang, Wenhao

作者机构:

关键词: quantitative trait loci;mapping;bacterial blight resistance;bulked segregant analysis;Oryza meyeriana

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )

ISSN: 1422-0067

年卷期: 2014 年 15 卷 7 期

页码:

收录情况: SCI

摘要: Oryza meyeriana is highly resistant to rice bacterial blight (BB) and this resistance trait has been transferred to cultivated rice (O. sativa) using asymmetric somatic hybridization. However, no resistance genes have yet been cloned. In the present study, a progeny of the somatic hybridization with high BB resistance was crossed with a rice cultivar with high BB susceptibility to develop an F-2 population. Using bulked segregant analysis (BSA), 17 polymorphic markers that were linked to rice BB resistance were obtained through scanning a total of 186 simple sequence repeats (SSR) and sequence-tagged site (STS) markers, evenly distributed on 12 chromosomes. A genetic linkage map was then constructed based on the 17 linkage markers and the F-2 segregating population, which was followed by mapping for quantitative trait loci (QTLs) for BB resistance. Three QTLs were identified on chromosomes 1, 3 and 5, respectively, and the alleles of the resistant parent at any of the QTLs increased BB resistance. All of the three QTLs had a strong effect on resistance, explaining about 21.5%, 12.3% and 39.2% of the resistance variance, respectively. These QTLs were different from the loci of the BB resistance genes that have been identified in previous studies. The QTLs mapped in this work will facilitate the isolation of novel BB resistance genes and their utilization in rice resistance breeding.

分类号:

  • 相关文献

[1]Use of asymmetric somatic hybridization for transfer of the bacterial blight resistance trait from Oryza meyeriana L. to O. sativa L. ssp japonica. Yan, CQ,Qian, KX,Yan, QS,Zhang, XQ,Xue, GP,Huangfu, WG,Wu, YF,Zhao, YZ,Xue, ZY,Huang, J,Xu, GZ,Wu, P. 2004

[2]Isolation and identification of a gene in response to rice blast disease in rice. Zheng, XW,Chen, XW,Zhang, XH,Lin, ZZ,Shang, JJ,Xu, JC,Zhai, WX,Zhu, LH.

[3]Quantitative Trait Loci Mapping for Chlorophyll Fluorescence and Associated Traits in Wheat (Triticum aestivum). Yang, De-Long,Jing, Rui-Lian,Chang, Xiao-Ping,Li, We.

[4]Identification of Quantitative Trait Loci for Bacterial Blight Resistance Derived from Oryza meyeriana and Agronomic Traits in Recombinant Inbred Lines of Oryza sativa. Chen, Li-Na,Yang, Yong,Yan, Cheng-Qi,Wang, Ming,Yu, Chu-Lang,Zhou, Jie,Cheng, Ye,Cheng, Xiao-Yue,Chen, Jian-Ping,Chen, Li-Na,Zhang, Wei-Lin,Cheng, Xiao-Yue. 2012

[5]Confirmation of a major QTL on chromosome 10 for maize kernel row number in different environments. Tian, Baohua,Wang, Jianhua,Wang, Guoying. 2014

[6]Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization. Zhu, YS,Chen, BT,Yu, SW,Zhang, DP,Zhang, XQ,Yan, QS.

[7]Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight. Wang, Xu-Ming,Zhou, Jie,Yang, Yong,Yu, Fei-Bo,Chen, Juan,Yu, Chu-Lang,Wang, Fang,Cheng, Ye,Yan, Cheng-Qi,Chen, Jian-Ping,Yu, Fei-Bo,Chen, Juan. 2013

[8]Identification and Genetic Analysis of a Novel Rice Spotted-Leaf Mutant with Broad-Spectrum Resistance to Xanthomonas oryzae pv. oryzae. Shen Hai-chao,Shi Yong-feng,Feng Bao-hua,Wang Hui-mei,Xu Xia,Huang Qi-na,Lu Xiang-guang,Wi Jian-li. 2014

[9]Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIA gamma 1. Jiang, GH,Xia, ZH,Zhou, YL,Wan, J,Li, DY,Chen, RS,Zhai, WX,Zhu, LH.

[10]Application of a simplified marker-assisted backcross technique for hybrid breeding in rice. Ji, Zhijuan,Zeng, Yuxiang,Qian, Qian,Yang, Changdeng,Shi, Jianyao,Ji, Zhijuan,Qian, Qian.

[11]Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza. Yi, Zili,Xiao, Youlun,Li, Jinjiang,Yu, Jianghui,Meng, Qiucheng,Deng, Xiangyang,Xiao, Guoying,Xiao, Youlun.

[12]Two RAPD markers linked to a major fertility restorer gene in pepper. Zhang, BX,Huang, SW,Yang, GM,Guo, JZ. 2000

[13]Effect of the puroindoline locus and environment on Chinese fresh noodle texture. Yang, EN,Zou, YC,Chen, DS,Sheppard, J,Martin, D,Huang, S,Mares, D,Sutherland, MW. 2006

[14]Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Huang, XQ,Wang, LX,Xu, MX,Roder, MS. 2003

[15]A Genome Scan for Quantitative Trait Loci Associated with Vibrio anguillarum Infection Resistance in Japanese Flounder (Paralichthys olivaceus) by Bulked Segregant Analysis. Wang, Lei,Wang, Lei,Fan, Caixia,Liu, Yang,Zhang, Yingping,Deng, Han,Xu, Ying,Tian, Yongsheng,Liao, Xiaolin,Xie, Mingshu,Li, Wenlong,Chen, Songlin,Wang, Lei,Liu, Shoutang,Sun, Deqiang.

[16]Bulked sample analysis in genetics, genomics and crop improvement. Zou, Cheng,Wang, Pingxi,Xu, Yunbi,Xu, Yunbi.

[17]QTL mapping of adult-plant resistance to stripe rust in a "Lumai 21xJingshuang 16' wheat population. He, Zhong H.,Xia, Xian C.,Ren, Yan,Liu, Li S.,He, Zhong H.,Wu, Ling,Bai, Bin.

[18]Genetic mapping of a putative Agropyron cristatum-derived powdery mildew resistance gene by a combination of bulked segregant analysis and single nucleotide polymorphism array. Lu, Yuqing,Wu, Xiaoyang,Yao, Miaomiao,Zhang, Jinpeng,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Du, Juan,Gao, Ainong,Li, Lihui.

[19]Characterization and fine mapping of a novel barley Stage Green-Revertible Albino Gene (HvSGRA) by Bulked Segregant Analysis based on SSR assay and Specific Length Amplified Fragment Sequencing. Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Qin, Dandan,Dong, Jing,Xu, Fuchao,Ge, Shuangtao,Xu, Qing,Li, Meifang,Guo, Ganggang,Xu, Yuxin. 2015

[20]The earlier identification of the seedless characteristic of the wampee [Clausena lansium (Lour.) Skeels] hybrid by a random amplified polymorphic DNA (RAPD) marker. Zhao Zhichang,Hu Guibing,Ou Yangruo,Liu Yunchun,Zhao Zhichang,Yi Yang,Zhao Zhichang,Chen Yeyuan. 2010

作者其他论文 更多>>