Genetic diversity and relationships among 47 loquat varieties revealed by EST-SSR markers

文献类型： 外文期刊

第一作者： Li, Xiao-ying

作者： Li, Xiao-ying;Xu, Hong-xia;Chen, Jun-wei

作者机构：

关键词： Genetic diversity;Relationship;Loquat;Varieties;EST-SSR;Marker

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2013 年 160 卷

页码：

收录情况： SCI

摘要： The genetic diversity and relationships of 47 loquat varieties sourced from different geographic locations were studied by using EST-SSR markers derived from apple. Results show that 33 pairs of apple EST-SSR primers had clear amplification products in loquat cultivars, with a comparatively high transferability rate of 67.3% in loquat, among which 22 pairs of primers could amplify polymorphic PCR products. Otherwise, among the 29 recovered fragments, 80% of the PCR products contained the SSR markers. The genetic relationships of all the 47 loquat accessions were analyzed by using polymorphic EST-SSR markers, showing that these markers could clearly separate loquat samples. Based on the cluster analysis, all the loquat varieties clustered into two groups with accessions from China and Spain respectively. The clusters obtained agree with the origins of the cultivars. This study confirms that the EST-SSR markers from apple are suitable for studying genetic diversity among loquat varieties. The polymorphic markers obtained will be used for loquat genotyping in further studies. (C) 2013 Elsevier B.V. All rights reserved.

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