Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas

文献类型： 外文期刊

第一作者： Wei, Qian

作者： Wei, Qian;Li, Jun;Zhang, Lin;Wu, Pingzhi;Chen, Yaping;Li, Meiru;Jiang, Huawu;Wu, Guojiang;Wei, Qian;Zhang, Lin;Li, Jun

作者机构：

关键词： Jatropha curcas;KAS II;Fatty acid

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2012 年 169 卷 8 期

页码：

收录情况： SCI

摘要： A cDNA clone encoding a putative beta-ketoacyl-acyl carrier protein (ACP) synthase II (KASII), a key enzyme in fatty acid biosynthesis, was isolated from Jatropha curcas L., a woody oil plant. The isolated cDNA clone of JcKASII contained a 1722-bp open reading frame coding for 573 amino acids with a predicted molecular mass of about 60.98 kDa and the conserved Cys(324) residues that has been proposed as the active site of KASII proteins. The deduced amino acid sequence of the cDNA clone had about 70-84% identity with the KASII from other plants. The transcript of JcKASII was detected in all tissues examined and increased during seed maturation. Expression of JcKASII in the Arabidopsis KASII mutant (fab1) could complement the fatty acid composition of the mutant. Overexpression of JcKASII cDNA under the cauliflower mosaic virus 35S promoter in Arabidopsis resulted in decreasing 16-carbon fatty acids and increasing 18-carbon fatty acids in leaves and seeds. Taken together, these results show that JcKASII could function in 18-carbon fatty acids accumulation in plant and may be useful in the genetic engineering of J. curcas. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.

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