Molecular cloning, expression, and regulation of goose leptin receptor gene in adipocytes

文献类型: 外文期刊

第一作者: Wang, Fang

作者: Wang, Fang;Fu, Yan;Wang, Fang;Lu, Lizhi;Yuan, Hao;Tian, Yong;Li, Jinjun;Shen, Junda;Tao, Zhengrong

作者机构:

关键词: Goose;Leptin receptor (LEPR);Expression;Regulation

期刊名称:MOLECULAR AND CELLULAR BIOCHEMISTRY ( 影响因子:3.396; 五年影响因子:3.282 )

ISSN: 0300-8177

年卷期: 2011 年 353 卷 1-2 期

页码:

收录情况: SCI

摘要: A full-length cDNA encoding the goose (Anser anser) leptin receptor (LEPR) was cloned and sequenced. The goose LEPR gene encodes a 1,156-amino acid protein containing a signal peptide, a single transmembrane domain and specific motifs involving putative leptin-binding and signal transduction. The deduced goose LEPR protein shows more than 90% identity to duck and 75% identity to chicken and turkey. Quantitative real-time analysis reveals that the goose LEPR is predominantly expressed in brain. The expression of LEPR in goose adipocytes can be up-regulated by oleic acid in vitro. Moreover, the expression levels of genes, which have been demonstrated to be related to adipocyte differentiation, are down-regulated in LEPR-knockdown adipocytes, indicating LEPR's potential role in adipocyte differentiation in goose.

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