Effects of linoleic acid and eicosapentaenoic acid on cell proliferation and lipid-metabolism gene expression in primary duck hepatocytes

文献类型： 外文期刊

第一作者： Liu, W. M.

作者： Liu, W. M.;Lu, L. Z.;Zhang, C.;Liu, Y. L.;Zhang, J.;Tao, Z. R.;Shen, J. D.;Li, G. Q.;Wang, D. Q.;Li, J. J.;Tian, Y.;Liu, W. M.;Shi, F. X.;Liu, Y. L.;Zhang, J.

作者机构：

关键词： Linoleic acid;Eicosapentaenoic acid;Proliferation;Hepatocyte;Duck

期刊名称：MOLECULAR AND CELLULAR BIOCHEMISTRY （ 影响因子：3.396； 五年影响因子：3.282 ）

ISSN： 0300-8177

年卷期： 2011 年 352 卷 1-2 期

页码：

收录情况： SCI

摘要： Several studies have investigated that linoleic acid (LA) and eicosapentaenoic acid (EPA) affect cell proliferation and lipid catabolic gene expression in mammals. To determine if LA and EPA increase duck cell proliferation and lipid catabolic gene expression, the authors exposed duck primary hepatocyte cultures to LA or EPA. The results showed that both LA and EPA increased cell proliferation in a dose-dependent manner (100 mu M). The effect on specific cell-cycle phases was also studied; LA and EPA (100 mu M) deceased the proportion of cells in the G0/G1 phase from 83 to 80.8 and 80.3%, respectively, concomitant with an increase in the proportion of S-phase cells (11.5 and 10.5 vs. 8%, respectively). The expression of PPAR-alpha and PPAR-alpha target genes, such as acyl-CoA oxidase (ACOX), lipoprotein lipase (LPL), liver fatty acid-binding protein (L-FABP), was examined by quantitative real-time PCR. The results showed that the expression of the PPAR-alpha, ACOX, and LPL genes increased significantly following LA and EPA exposure, but that the expression of L-FABP remained unchanged. This study provides the first characterization of LA- and EPA-induced cell proliferation and PPAR-alpha and PPAR-alpha target gene transcriptional responses in duck primary hepatocyte cultures.

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