Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase

文献类型： 外文期刊

第一作者： Li, Yin

作者： Li, Yin;Liu, Zhiqiang;Liu, Zhiqiang;Li, Geng;Cui, Fengjie;Ping, Lifeng;Qiu, Chongyan;Yan, Lijiao;Yan, Lijiao

作者机构：

关键词： Alternaria sp;ND-16;Isolation;Identification;Production;Xylanase

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2009 年 157 卷 1 期

页码：

收录情况： SCI

摘要： Alternaria sp. ND-16, a bacterium isolated from soil sample, was identified as a strain of Alternaria mali based on the morphology and comparison of internal transcribed spacer rDNA gene sequence studies. Furthermore, it is demonstrated that this strain has xylanase activity, and the activity can be optimized under suitable growing conditions where wheat bran and urea are the primary sources of carbon and nitrogen. Partially purified xylanase from Alternaria sp. ND-16 is shown to have an optimal pH of 6.0 and optimal temperature of 50 A degrees C, making this enzyme potentially suitable for industrial applications. It is also demonstrated that Na(+) and Mn(2+) show strong inhibition of the xylanase while K(+), Li(+), Fe(2+), Cu(2+), and Zn(2+) have no significant effect on the activity.

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