Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes

文献类型： 外文期刊

第一作者： Huang, Jing

作者： Huang, Jing;Li, Qingwang;Li, Wenye;Xiao, Bo;Wu, Shuyun;Jiang, Zhongliang;Hu, Jianhong;Li, Qingwang;Zhao, Rui;Han, Zengsheng;Liu, Lei;Chen, Xiaoyu;Xiao, Bo

作者机构：

关键词： porcine;oocyte;vitrification;maturation

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.145； 五年影响因子：2.281 ）

ISSN： 0378-4320

年卷期： 2008 年 106 卷 1-2 期

页码：

收录情况： SCI

摘要： This study examined the effects of monosaccharide (glucose), disaccharide (sucrose) and polysaccharides (Ficoll and Lycium barbarum polysaccharide (LBP)) at different concentrations, using ethylene glycol (EG) as membrane-permeating cryoprotectant, on in vitro maturation of vitrified-thawed immature (GV) porcine oocytes. A total of 1145 oocytes were obtained by follicle aspiration from 496 ovaries of pigs slaughtered at a local abattoir and vitrified using a five-step method. After thawing and removal of cryoprotectant, oocytes were cultured for 44h at 39 degrees C in a humidified atmosphere of 5% CO2 in air. Oocytes were stained with DAPI and nuclear maturation was examined. The highest maturation rates were obtained in 1.5 M glucose (8.62%), 0.75 M sucrose (20.0%), 3.0 g/ml Ficoll (13.79%) and 0.10 g/ml LBP (20.69%), respectively. The maturation rate using 0.75 M sucrose or 0.10 g/ml LBP was significantly higher compared to 1.5 M glucose (P < 0.05), but there was no significant difference from using 3.0 g/ml Ficoll (P > 0.05). The percentage of oocytes reaching metaphase H (MII) stage in the cryopreserved groups was significantly lower than control (P < 0.05). These results suggest that LBP is an effective non-permeating membrane cryoprotectant and 0.75 M sucrose or 0.10 g/ml LBP can be used as the vitrification solution for immature porcine oocytes. (c) 2007 Elsevier B.V. All rights reserved.

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