Duplex SYBR Green I real-time RT-PCR for Simultaneous Detection of Goose Astrovirus Genotypes 1 and 2

文献类型： 外文期刊

第一作者： Zhai, Qi

作者： Zhai, Qi;Zhao, Liang;Jia, Chun-Ling;Zhou, Xiu-Rong;Luo, Sheng-Jun;Wen, Xiao-Hui;Lv, Dian-Hong;Zhai, Qi;Zhai, Qi;Jia, Chun-Ling;Zhou, Xiu-Rong;Luo, Sheng-Jun;Wen, Xiao-Hui;Lv, Dian-Hong;Zhai, Qi;Jia, Chun-Ling;Zhou, Xiu-Rong;Luo, Sheng-Jun;Wen, Xiao-Hui;Lv, Dian-Hong

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关键词： Goose astrovirus genotype 1 and 2 (GAstV 1 and GAstV 2); Duplex SYBR Green I realtime RT-PCR (qRT-PCR); Diagnostic tool; Waterfowl farming

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：2.3； 五年影响因子：1.5 ）

ISSN： 0253-8318

年卷期： 2023 年 43 卷 4 期

页码：

收录情况： SCI

摘要： As the prevalence and complexity of goose astrovirus genotype 1 and 2 (GAstV 1 and GAstV 2) infections continue to rise in China's goose farming industry, a rapid, reliable and cost-effective diagnostic tool is urgently needed. Duplex SYBR Green I real-time RT-PCR (qRT-PCR) method was developed, which is capable of simultaneous detection of GAstV 1 and GAstV 2. We designed two primer pairs, targeting the viruses conserved genomic regions, facilitating the distinction of GAstV 1 and GAstV 2 through unique melting curve peaks. This method exhibited strong linear relationships in standard curves (R2 = 0.99), and detection limits were 3.18 x 102 and 3.16 x 102 copies/mu L for GAstV 1 and GAstV 2, respectively. With intra- and inter-assay variation below 2%, the assay exhibits excellent repeatability. This duplex qRT-PCR, displaying consistent detection rates comparable to a TaqMan-based method, offers a promising, rapid, and cost-effective diagnostic strategy for waterfowl farming.

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