Isolation and identification of a novel Rhodococcus sp ML-0004 producing epoxide hydrolase and optimization of enzyme production

文献类型： 外文期刊

第一作者： Liu, Zhiqiang

作者： Liu, Zhiqiang;Li, Yin;Ping, Lifeng;Xu, Yingying;Cui, Fengjie;Xue, Yaping;Zheng, Yuguo

作者机构：

关键词： Rhodococcus opacus ML-0004;epoxide hydrolase;L.(+)-tartaric acid;isolation;identification

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2007 年 42 卷 5 期

页码：

收录情况： SCI

摘要： Rhodococcus sp. ML-0004, a novel strain for producing epoxide hydrolase, was isolated from soil in this study. The epoxide hydrolase can catalyze the stereo-specific hydrolysis of cis-epoxysuccinic acid to generate L(+)-tartaric acid. By examining physiological, biochemical characteristics and comparing its 16S rDNA gene sequence, it was identified as Rhodococcus opacus, and named R. opacus ML-0004. The optimal conditions for epoxide hydrolase production from R. opacus ML-0004 were also investigated. Propanediol and (NH4)(2)SO4 were selected as carbon source and nitrogen source, respectively, for the production of R. opacus ML-0004 epoxide hydrolase. The optimal conditions for epoxide hydrolase production were fermentation temperature = 28 degrees C, pH 7.0, and cultivation time = 26 h. Under these conditions, the maximum epoxide hydrolase activity reached 10.5 U mL(-1). (C) 2007 Elsevier Ltd. All rights reserved.

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