Characterization of three-spot seahorse (Hippocampus trimaculatus) skin collagen and its fibrillar gel reinforced by EDC: A comparative study
文献类型: 外文期刊
第一作者: Liao, Wei
作者: Liao, Wei;Liu, Yangfeng;Xia, Guanghua;Shen, Xuan Ri;Duan, Zhouwei;Yang, Jian
作者机构:
关键词: collagen;EDC;gel-forming capacity;thermal stability;three-spot seahorse
期刊名称:JOURNAL OF FOOD BIOCHEMISTRY ( 影响因子:2.72; 五年影响因子:2.543 )
ISSN: 0145-8884
年卷期: 2017 年 41 卷 5 期
页码:
收录情况: SCI
摘要: In current study, three-spot seahorse (Hippocampus trimaculatus) skin collagen (SC) was extracted for the first time, and its physicochemical properties were characterized. Nile tilapia-skin collagen (TC) and pig-skin collagen (PC) were used to the references. The yields of SC and TC were 40.41 +/- 1.23 and 52.6 +/- 0.97% (dry weight), respectively. The SDS-PAGE and FTIR confirmed that SC, TC, and PC were preliminarily identified as type I collagen with triple helical structure. The denaturation temperatures of SC, TC, and PC were 35.12, 38.66, and 42.56 degrees C, respectively. Gelforming studies indicated SC exhibited higher capability than TC and PC under the effect of NaCl. After cross-linking by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), SC showed a significant increase in thermal stability and mechanical strength, and electron microscopic observation corroborated this phenomenon. Therefore, the isolated collagen from three-spot seahorse skin can be used in food packaging and biomaterials as alternatives to mammalian collagen. Practical applications In this study, we evaluated a new source of skin collagen from three-spot seahorse which has been large-scale artificially cultured in recent years. The finding of this study demonstrate that SC exhibited higher gel-forming capability under the effect of NaCl, which suggest that it may be used in photographic film, sausage casings, and food additive as alternatives to mammalian collagen. Furthermore, thermal stability and mechanical strength of SC gel have a significant increase after cross-linking by EDC, so it can be further used for biomaterials, such as food packaging, drug delivery, tissue engineering constructs, and wound dressing systems.
分类号:
- 相关文献
作者其他论文 更多>>
-
Two homoeoallelic gene expression of TaCHLIs ensures normal chlorophyll biosynthesis in Hexaploid wheat
作者:Zhao, Yankun;Ban, Jinfu;Li, Zhankun;Li, Caihua;Fu, Xiaoyi;Gao, Xinmei;Ding, Xin;He, Mingqi;Gao, Zhenxian;Yang, Jian;Zhang, Yu'e;Wang, Yan;Hu, Weiguo;Wang, Xicheng;Zhou, Yanjie;Cao, Tingjie;Zou, Yanmin;Yang, Junfeng;Zhang, Wensheng
关键词:Magnesium chelatase; Genetic complementarity; Hexaploid wheat; Homoeologs loss; Redundant mRNA dosage
-
Ubiquitination of OsCSN5 by OsPUB45 activates immunity by modulating the OsCUL3a-OsNPR1 module
作者:Zhang, Chongyang;Fang, Liang;He, Feng;You, Xiaoman;Wang, Min;Zhao, Tianxiao;Hou, Yanyan;Wang, Ruyi;Ning, Yuese;Zhang, Chongyang;Ruan, Jue;Fang, Liang;Francis, Frederic;Xiao, Ning;Li, Aihong;Yang, Jian;Wang, Guo-Liang
关键词:
-
Antiviral activity of Morus alba L. . extract against pseudorabies virus
作者:Zhang, Xiaoai;Yang, Jian;Mo, Minying;Farooq, Muhammad;Li, Jianbo;Wei, Wenkang;Yao, Chunpeng;Yang, Jian;Liu, Fan
关键词:Antiviral activity; Morus alba; Ethanol extract; Pseudorabies virus
-
The Extract of Piper nigrum Improves the Cognitive Impairment and Mood in Sleep-Deprived Mice Through the JAK1/STAT3 Signalling Pathway
作者:Guan, Dongyan;Hou, Zhiying;Fan, Bei;Bai, Yajuan;Wu, Honghong;Yu, Jiawei;Wang, Fengzhong;Wang, Qiong;Guan, Dongyan;Wang, Qiong;Xie, Hui;Duan, Zhouwei
关键词:piper extract; network pharmacology; sleep deprivation; cognitive impairment
-
Transcriptional Analysis of Spodoptera frugiperda Sf9 Cells Infected with Daphnis nerii Cypovirus-23
作者:Kuang, Wendong;Yang, Jian;Wang, Jinchang;Chen, Junhui;Zhan, Zhigao;Guan, Limei;Li, Jianghuai;Deng, Tao;Jin, Liang;Kuang, Wendong;Yang, Jian;Wang, Jinchang;Chen, Junhui;Yang, Chunhua;Zhan, Zhigao;Guan, Limei;Li, Jianghuai;Yang, Feiying;Jin, Liang;Kuang, Wendong;Yan, Chenghua;Ma, Guangqiang;Liu, Xinsheng
关键词:
Daphnis nerii cypovirus-23; Sf9 cells; transcriptome analysis -
Enhancing methane cracking through variable-frequency microwave heating: Improved energy efficiency and carbon nanotube formation
作者:Liu, Haolin;Liao, Wei;Wen, Mengyuan;Wang, Jia;Jiang, Jianchun;Ren, Jurong;Wang, Jia;Jiang, Jianchun;Wang, Shule;Meng, Xianzhi;Xiong, Lingming;Jin, Yanghao
关键词:Microwave frequency; Simulation; Methane cracking; Carbon nanotubes
-
Structural and functional analysis of soy protein-skipjack tuna protein gels prepared by two-screw extrusion and their effects on metabolites and gut microbiota in vitro
作者:Zhang, Zhihong;Liu, Fanrui;Chen, Yubei;Li, Baolian;Feng, Ying;Wang, Honglan;He, Hai;Gao, Yang;Xia, Guanghua;He, Hai
关键词:Skipjack tuna fish protein; Soy protein; Twin-screw extrusion; Gelation behavior; Fecal fermentation