Cherry Valley Ducks Mitochondrial Antiviral-Signalling Potein-Mediated Signaling Pathway and Antiviral Acitvity Research

文献类型: 外文期刊

第一作者: Li, Ning

作者: Li, Ning;Hong, Tianqi;Li, Rong;Wang, Yao;Guo, Mengjiao;Cai, Yumei;Liu, Sidang;Chai, Tongjie;Wei, Liangmeng;Li, Ning;Chai, Tongjie;Wei, Liangmeng;Cao, Zongxi

作者机构:

关键词: cherry valley duck;MAVS;molecular cloning;signaling pathway;antiviral ability

期刊名称:FRONTIERS IN IMMUNOLOGY ( 影响因子:7.561; 五年影响因子:7.624 )

ISSN: 1664-3224

年卷期: 2016 年 7 卷

页码:

收录情况: SCI

摘要: Mitochondrial antiviral-signaling protein (MAVS), an adaptor protein of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs)-mediated signal pathway, is involved in innate immunity. In this study, Cherry Valley duck MAVS (duMAVS) was cloned from the spleen and analyzed. duMAVS was determined to have a caspase activation and recruitment domain at N-terminal, followed by a proline-rich domain and a transmembrane domain at C-terminal. Quantitative real-time PCR indicated that duMAVS was expressed in all tissues tested across a broad expression spectrum. The expression of duMAVS was significantly upregulated after infection with duck Tembusu virus (DTMUV). Overexpression of duMAVS could drive the activation of interferon (IFN)-beta, nuclear factor-kappa B, interferon regulatory factor 7, and many downstream factors (such as Mx, PKR, OAS, and IL-8) in duck embryo fibroblast cells. What is more, RNA interference further confirmed that duMAVS was an important adaptor for IFN-beta activation. The antiviral assay showed that duMAVS could suppress the various viral replications (DTMUV, novel reovirus, and duck plague virus) at early stages of infection. Overall, these results showed that the main signal pathway mediated by duMAVS and it had a broad-spectrum antiviral ability. This research will be helpful to better understanding the innate immune system of ducks.

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