Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus

文献类型: 外文期刊

第一作者: Wen, Guoyuan

作者: Wen, Guoyuan;Yang, Jun;Luo, Qingping;Liao, Yonghong;Zhang, Rongrong;Wang, Hongling;Ai, Diyun;Luo, Ling;Shao, Huabin;Zhang, Tao;Wen, Guoyuan;Hu, Zhibin;Song, Nianhua;Zhang, Tao

作者机构:

关键词: Classical swine fever virus;Real-time RT-PCR;Differential diagnosis;Wild-type strain;HCLV strain

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2011 年 176 卷 1-2 期

页码:

收录情况: SCI

摘要: A one-step real-time RT-PCR assay using a minor groove binding probe was developed for the specific detection of Chinese wild-type classical swine fever virus (CSFV). The assay detected wild-type CSFV strains representing different genotypes, but did not amplify viral RNA from the Hog Cholera Lipinized Virus (HCLV) vaccine-strain and other porcine viruses. The assay had a detection limit of 10 copies/reaction or 3.0 median tissue culture infective dose/reaction. In comparison to the sequencing nested RT-PCR assay, the sensitivity and specificity of the assay were 98.3% and 94.3%. respectively, when testing 515 veterinary samples. Wild-type CSFV RNA was detected in nasal swabs 2-4 days before detection in serum samples from pigs exposed to infection by contact. and 2-4 days prior to the onset of clinical disease. HCLV RNA remained undetectable in nasal swabs and serum samples from vaccinated pigs. In conclusion, the novel assay described in this study provides a rapid and sensitive method for differentiating between wild-type and the HCLV-strain of CSFV. It could be used for monitoring in CSF outbreak areas or as a screening method for CSFV eradication strategies. (C) 2011 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]A multiplex nested RT-PCR for the detection and differentiation of wild-type viruses from C-strain vaccine of classical swine fever virus. Li, Yan,Zhao, Jian-Jun,Li, Na,Shi, Zixue,Cheng, Dan,Zhu, Qing-Hu,Tu, Changchun,Tong, Guang-Zhi,Qiu, Hua-Ji. 2007

[2]Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs. Yi, Li,Cheng, Shipeng,Xu, Hongli,Wang, Jianke,Cheng, Yuening,Yang, Shen,Luo, Bin. 2012

[3]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[4]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[5]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[6]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

[7]Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus. Tong, Guang-Zhi,Bin Wang,Hua, Rong-Hong,Tian, Zhi-Jun,Chen, Na-Sha,Zhao, Fu-Rong,Liu, Tian-Qiang,Wang, Yun-Feng,Tong, Guang-Zhi. 2009

[8]An efficient method of noroviruses recovery from oysters and clams. Zhou Deqing,Ma Liping,Zhao Feng,Yao Lin,Li Xinguang,Ma Liping,Su Laijin,Li Xinguang. 2013

[9]Differentially expressed genes under cold acclimation in Physcomitrella patens. Sun, Ming-Ming,Li, Lin-Hui,Xie, Hua,Ma, Rong-Cai,He, Yi-Kun. 2007

[10]Tissue distribution and expression of signaling lymphocyte activation molecule receptor to peste des petits ruminant virus in goats detected by real-time PCR. Meng, Xuelian,Dou, Yongxi,Zhai, Junjun,Zhang, Hairui,Yan, Fengchao,Shi, Xiaoni,Luo, Xuelong,Li, Hui,Cai, Xuepeng. 2011

[11]Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus. Yun, Tao,Ni, Zheng,Hua, Jionggang,Ye, Weicheng,Chen, Liu,Zhang, Shuai,Zhang, Yan,Zhang, Cun,Zhang, Shuai. 2012

[12]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

[13]Pathogenesis of a genotype C strain of bovine parainfluenza virus type 3 infection in albino guinea pigs. Shi, Hong-Fei,Zhu, Yuan-Mao,Dong, Xiu-Mei,Cai, Hong,Ma, Lei,Wang, Shu,Yan, Hao,Wang, Xue-Zhi,Xue, Fei.

[14]Relation Between the Viral Load Accumulation of Southern Rice Black-Streaked Dwarf Virus and the Different Developmental Stages of Sogatella furcifera (Hemiptera: Delphacidae). Hou, Mao-Lin,Liu, Yu-Di.

[15]Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses. Wang Xiu-rong,Gu Lin-lin,Shi Jian-zhong,Xu Hai-feng,Zhang Ying,Zeng Xian-ying,Deng Guo-hua,Li Cheng-jun,Chen Hua-lan. 2017

[16]Outbreak of Porcine Epidemic Diarrhea in Piglets in Gansu Province, China. Wang, Hui,Xia, Xinchao,Liu, Yongming,Wang, Shengyi,Qi, Zhiming,Liu, Shixiang,Wang, Haijun,Liu, Zhiqi,Niu, Xu,Liu, Shanshan. 2013

[17]The survey of porcine teschoviruses in field samples in China with a universal rapid probe real-time RT-PCR assay. Zhang, Chaofan,Wang, Ming,Zhang, Chaofan,Hu, Feng,Qiu, Zheng,Cui, Shangjin,Wang, Zhongtian,Liu, Yebing,Zhou, Shun.

[18]Screening of valid reference genes for real-time RT-PCR data normalization in Hevea brasiliensis and expression validation of a sucrose transporter gene HbSUT3. Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Xiao, Xiaohu. 2011

[19]Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Gu, Chaojiang,Zheng, Congyi,Shi, Lili,Zhang, Qian,Li, Yong,Lu, Bin,Xiong, Yi,Qu, Sanpu,Shao, Junjun,Chang, Huiyun. 2007

[20]The presence of Genogroup II Norovirus in Retail Shellfish from Seven Coastal Cities in China. Ma, Li-ping,Zhao, Feng,Yao, Lin,Li, Xin-guang,Zhou, De-qing,Zhang, Rui-ling,Ma, Li-ping.

作者其他论文 更多>>