Characterization and bioactivity of water-soluble polysaccharides from the fruit of pumpkin

文献类型： 外文期刊

第一作者： Wang, Yunqiang

作者： Wang, Yunqiang;Zhang, De;Xiang, Changping;Li, Junli;Wang, Yunqiang;Hu, Xiaohua;Zhang, Zhengqin

作者机构：

关键词： Pumpkin polysaccharides;characterization;antioxidant activities

期刊名称：JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT （ 影响因子：0.435； 五年影响因子：0.484 ）

ISSN： 1459-0255

年卷期： 2010 年 8 卷 2 期

页码：

收录情况： SCI

摘要： Four fractions of water-soluble polysaccharide, coded as PP1, PP2, PP3 and PP4, were isolated and purified from the fruit of pumpkin at 20 degrees C, 40 degrees C, 60 degrees C and 80 degrees C, respectively. Their chemical structure and physico-chemical properties were characterized by FT-IR spectra, gas chromatography and viscometry, respectively. Furthermore, their antioxidant activities were evaluated in vitro. The results indicated that four fractions of pumpkin polysaccharide were all composed of L-arabinose (Ara), L-rhamnose (Rha), D-glucose (Glu), D-galactose (Gal) and D-glucuronic acid, with variation of the component ratio. The polysaccharides showed polyelectrolyte effect due to the existing of glucuronic acid. PP1 showed the highest scavenging rate on hydroxyl radicals and superoxide radicals among four fractions of pumpkin polysaccharide. The four polysaccharide fractions were found to have the ability to alleviate the liver mitochondria swelling at concentration 2 mg/ml. The scavenging rate on lipid peroxidation in liver homogenate by anti-oxidation of PP1, PP2, PP3 and PP4 were 64.09%, 55.3%, 35.4% and 49.9% at concentration 2.5 mg/ml, respectively.

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