Molecular cloning and polymorphism of the porcine H2AFZ gene

文献类型： 外文期刊

第一作者： Zhang, Y. H.

作者： Zhang, Y. H.;Zuo, B.;Lei, M. G.;Xiong, Y. Z.;Li, F. E.;Zhang, Y. H.;Zuo, B.;Lei, M. G.;Xiong, Y. Z.;Li, F. E.;Mei, S. Q.;Peng, X. W.

作者机构：

关键词： pig;H2AFZ gene;litter size;PCR-Bsu15l-RFLP;ovary

期刊名称：ANIMAL （ 影响因子：3.24； 五年影响因子：3.223 ）

ISSN： 1751-7311

年卷期： 2009 年 3 卷 6 期

页码：

收录情况： SCI

摘要： H2A histone family, member Z (H2A.Z) is required for early mammalian development. In the present study, the 932 by of full-length cDNA encoding a 128 amino-acid protein and the sequences of intron 2 to 4 of the porcine H2A histone family, member Z (pH2AFZ) gene were obtained. By comparative sequencing of pH2AFZ gene in Large White and Meishan pigs, a 4 by deletion/insertion in intron 2 was detected and a PCR-Bsu15l-RFLP was established to detect this variation. In DIV (4th Dam line of Chinese lean-type new lines) pigs, the first-parity females with AA genotype had fewer piglets born alive (-2.64 and -1.83 piglets per litter) than those with AB (P < 0.01) and BB (P < 0.05) genotype. The additive allelic and dominance effect were estimated to be 0.92 (P < 0.05) and -0.87 piglets per litter (P < 0.01) for number of piglets born alive, respectively. This result suggests that the pH2AFZ gene might be a good candidate gene of litter-size trait and provides some marker information for marker-assisted selection.

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