文献类型： 外文期刊

第一作者： Gao Hong

作者： Gao Hong;Gu Wen-Ying

作者机构：

关键词： Agaricus brasiliensis;ergosterol;triple-wavelength spectrophotometry

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2007 年 35 卷 4 期

页码：

收录情况： SCI

摘要： The ergosterol content in mycelia of Agaricus brasiliensis was determined by triple-wavelength spectrophotometry. Ergosterol was separated from other impurities using TLC method. According to the UV scan curve of ergosterol and impurities, the triple wavelengths were identified as 272 nm, 282 nm and 292 nm. The value of AA is only relevant to the ergosterol concentration. Therefore it can eliminate the absorbance error caused by interfering components. The regression equation of concentration vs Delta A was obtained : Delta A = 0.00403C + 0.001048 with a correlation coefficient of r = 0.9996, and a linear relationship was obtained in the ergosterol concentrations of 0-50 mg/L with Delta A. The relative standard deviation (RSD) of precision test was 1.2% (n=5) and the recovery of standard addition is 97.5% - 102.5%. The result of the method is close to that of HPLC method. The method is easier and cheaper than HPLC method, and more accurate than single-wavelength spectrophotometry.

分类号：