Identification of integration of foreign gene in transgenic pigs

文献类型: 外文期刊

第一作者: Liu, GS

作者: Liu, GS;Chen, QX;Jing, W

作者机构:

关键词: porcine growth hormone gene;PCR;DNA blotting;transgenic pig

期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )

ISSN: 1000-3282

年卷期: 1996 年 23 卷 3 期

页码:

收录情况: SCI

摘要: Foreign porcine growth hormone genes were introduced into Hubei white pigs. In order to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection, The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration.

分类号:

  • 相关文献

[1]Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer. Pan DengKe,Zhang Li,Feng Chong,Long Chuan,Liu Xiao,Zhou YanRong,Chen HongXing,Wan Rong,Zhang Jian,Lin AiXing,Dong EnQiu,Wang ShuChen,Xu HouGang. 2010

[2]Safety assessment of sFat-1 transgenic pigs by detecting their co-habitant microbe in intestinal tract. Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Tang, Maoxue,Cheng, Wenke,Jin, Erhui,Yang, Shulin,Cui, Wentao,Li, Kui,Zheng, Xinmin,Chen, Hongxing.

[3]Comprehensive molecular characterization of a transgenic pig expressing hCD46 gene. Wang, Qin,Liu, Xiaofei,Fu, Wei,Lin, Xiangmei,Shi, Ningning,Pan, Dengke,Shi, Ningning,Shang, Ying,Shang, Ying,Xu, Wentao,Zhao, Yiqiang.

[4]Rapid and Sensitive Detection of sFAT-1 Transgenic Pigs by Visual Loop-Mediated Isothermal Amplification. Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Zhou, Rong,Zheng, Xinmin,Ren, Hongyan.

[5]The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae. Zhou, Yanrong,Lin, Yanli,Wu, Xiaojie,Xiong, Fuyin,Chen, Hongxing,Feng, Chong,Long, Chuan,Wang, Ning,Pan, Dengke. 2014

[6]Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs. Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Zheng, X. M.,Hou, J..

[7]Resistance to eyespot of wheat, caused by Tapesia yallundae, derived from Thinopyrum intermedium homoeologous group 4 chromosome. Li, HJ,Arterburn, M,Jones, SS,Murray, TD. 2005

[8]Comparative analysis of different protocols for extraction of DNA from fish scales of Cyprinus carpio. Li, Yanhe,Gul, Yasmeen,Cui, Lei,Cao, Xiaojuan,Wang, Weimin,Li, Yanhe,Gul, Yasmeen. 2015

[9]Development and application of a loop-mediated isothermal amplification method for rapid detection of Haemophilus parasuis. Zhang, Jian-Min,Xu, Cheng-Gang,Guo, Li-Li,Zhang, Bin,Li, Jing-Yi,Chen, Ji-Dang,Fan, Hui-Ying,Liao, Ming,Shen, Hai-Yan. 2011

[10]Loop-Mediated Isothermal Amplification Assay Targeting the MOMP Gene for Rapid Detection of Chlamydia psittaci Abortus Strain. Lin, Guo-Zhen,Zheng, Fu-Ying,Zhou, Ji-Zhang,Wang, Guang-Hua,Cao, Xiao-An,Gong, Xiao-Wei,Qiu, Chang-Qing. 2012

[11]First report of the molecular detection of Ancylostoma caninum in Lahore, Pakistan: the threat from pets. Rehman, A.,Shehzad, W.,Islam, S.,Bajwa, A. A.,Akhtar, R.,Rashid, I.,Akbar, H.,Riaz, F.,Waqas, M.. 2017

[12]Molecular investigation of piroplasma infection in white yaks (Bos grunniens) in Gansu province, China. Li, Sitong,Liu, Junlong,Liu, Aihong,Li, Youquan,Yin, Hong,Luo, Jianxun,Guan, Guiquan,Wang, Shuqing,Wang, Shufang,Yin, Hong. 2017

[13]Negative evidence of parthenogenesis induction by Wolbachia in a gallwasp species, Dryocosmus kuriphilus. He, Yi-Yuan,Fan, Yong-Sheng,Ma, Ming-Yong,Peng, De-Liang. 2007

[14]Genetic Algorithm Applied to the Selection of Factors in Principal Component: ASQR study of Aromatic Hydrocarbons Toxicity to Chlorella vulgaris. Yang Sheng-Long,Wang Cui-Hua,Wu Yang,Yu Hong-Xia,Wang Lian-Shen. 2013

[15]A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations. Geng, Lige,Deng, Dewayne D.,Wubben, Martin J.,Jenkins, Johnie N.,McCarty, Jack C., Jr.,Abdurakhmonov, Ibrokhim. 2017

[16]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

[17]Successful detection of foreign inserts in transgenic rice TT51-1 (BT63) by RNA-sequencing combined with PCR. Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Huang, Shunmou,Wu, Gang,Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Wu, Gang.

[18]Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China. Pei, Shi-Chun,Zhang, Hong-Fu,Ji, Cheng,Pei, Shi-Chun,Zhen, Yu-Ping,Gao, Jian-Wei,Lee, Won-Jong,Chen, Cong,Zhang, Xuan-Zhe.

[19]Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization. Miao, W,Yu, T,Orias, E,Wan, ML,Fu, CJ.

[20]An epidemiological survey of Theileria infections in small ruminants in central China. Li, Youquan,Zhang, Xiao,Liu, Zhijie,Chen, Ze,Yang, Jifei,He, Haining,Guan, Guiquan,Liu, Aihong,Ren, Qiaoyun,Niu, Qingli,Liu, Junlong,Luo, Jianxun,Yin, Hong,Li, Youquan,Zhang, Xiao,Liu, Zhijie,Chen, Ze,Yang, Jifei,He, Haining,Guan, Guiquan,Liu, Aihong,Ren, Qiaoyun,Niu, Qingli,Liu, Junlong,Luo, Jianxun,Yin, Hong,Li, Youquan,Zhang, Xiao,Liu, Zhijie,Chen, Ze,Yang, Jifei,He, Haining,Guan, Guiquan,Liu, Aihong,Ren, Qiaoyun,Niu, Qingli,Liu, Junlong,Luo, Jianxun,Yin, Hong.

作者其他论文 更多>>