Identification of integration of foreign gene in transgenic pigs
文献类型: 外文期刊
第一作者: Liu, GS
作者: Liu, GS;Chen, QX;Jing, W
作者机构:
关键词: porcine growth hormone gene;PCR;DNA blotting;transgenic pig
期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )
ISSN: 1000-3282
年卷期: 1996 年 23 卷 3 期
页码:
收录情况: SCI
摘要: Foreign porcine growth hormone genes were introduced into Hubei white pigs. In order to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection, The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration.
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