miR397/Laccase Gene Mediated Network Improves Tolerance to Fenoxaprop-P-ethyl in Beckmannia syzigachne and Oryza sativa

文献类型： 外文期刊

第一作者： Zhao, Hongwei

作者： Zhao, Hongwei;Dong, Liyao;Pan, Lang;Zhao, Hongwei;Dong, Liyao;Yu, Qin;Bai, Lianyang

作者机构：

关键词： Beckmannia syzigachne;fenoxaprop-P-ethyl-resistance;non-target-site resistance;miR397;Laccase;metabolism

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Herbicide resistance can be either target-site or non-target-site based. The molecular mechanisms underlying non-target-site resistance (NTSR) are poorly understood, especially at the level of gene expression regulation. MicroRNAs (miRNAs) represent key post-transcriptional regulators of eukaryotic gene expression and play important roles in stress responses. In this study, the miR397 gene from Beckmannia syzigachne (referred to as bsy-miR397) was functionally characterized to determine its role in regulating fenoxaprop-P-ethyl resistance. We showed that (1) bsy-miR397 transcript level is constitutively higher in resistant than in sensitive B. syzigachne plants, whereas bsy-Laccase expression and activity show the opposite trend, and (2) bsy-miR397 suppresses the expression of bsy-Laccase in tobacco, indicating that it negatively regulates bsy-Laccase at the transcriptional level. We found evidences that miR397/laccase regulation might be involved in fenoxaprop-P-ethyl NTSR. First, the rice transgenic line overexpressing OXmiR397 showed improved fenoxaprop-P-ethyl tolerance. Second, following activation of bsy-Laccase gene expression by CuSO4 treatment, fenoxaprop resistance in B. syzigachne tended to decrease. Therefore, we suggest that bsy-miR397 might play a role in fenoxaprop-P-ethyl NTSR in B. syzigachne by down-regulating laccase expression, potentially leading to the enhanced expression of three oxidases/peroxidases genes to introduce an active moiety into herbicide molecules in Phase-2 metabolism. Bsy-miR397,bsy-Laccase, and other regulatory components might form a regulatory network to detoxify fenoxaprop-P-ethyl in B. syzigachne, supported by the differential expression of transcription factors and oxidases/peroxidases in the rice transgenic line overexpressing OXmiR397. This implies how down-regulation of a gene (laccase) can enhance NTSR. Our findings shed light on the daunting task of understanding and managing complex NTSR in weedy plant species.

分类号：

  • 相关文献

[1]Exploring laccase genes from plant pathogen genomes: a bioinformatic approach. Peng, B. Z.,Li, P. Q.,Fu, L.,Yu, X. M.. 2015

[2]Effect of Supplements Mn2+, Cu2+, and Aromatic Compounds and Penicillium decumbens on Lignocellulosic Enzyme Activity and Productivity of Catathelasma ventricosum. Sun, Jun,Yang, Yanjun,Liu Yuntao,Sun, Jun,Su, Yujie,Yang, Yanjun,Luo, Zeyu,Rao, Shengqi. 2013

[3]Heterologous expression and characterisation of a laccase from Colletotrichum lagenarium and decolourisation of different synthetic dyes. Wang, Bo,Tian, Yongsheng,Zhao, Wei,Li, Zhengjun,Gao, Jianjie,Peng, Rihe,Yao, Quanhong,Yan, Ying.

[4]Purification and characterization of a laccase from Coprinopsis cinerea in Pichia pastoris. Wang, Bo,Wang, Lijuan,Lin, Yaqiu,Han, Jing,Gao, Jianjie,Tian, Yongsheng,Zhao, Wei,Peng, Rihe,Yao, Quanhong,Wang, Bo,Wang, Lijuan,Han, Jing,Gao, Jianjie,Tian, Yongsheng,Zhao, Wei,Peng, Rihe,Yao, Quanhong,Lin, Yaqiu,Han, Qing,Han, Qing.

[5]Enhanced Performance of Magnetic Graphene Oxide-Immobilized Laccase and Its Application for the Decolorization of Dyes. Chen, Jing,Leng, Juan,Yang, Xiai,Liao, Liping,Liu, Liangliang,Xiao, Aiping.

[6]Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Zou, Ya-Jie,Huang, Chen-Yang,Zhang, Jin-Xia,Wang, He-Xiang,Ng, Tzi-Bun.

[7]Decolorization of Alizarin Red and other synthetic dyes by a recombinant laccase from Pichia pastoris. Zheng, Miaomiao,Shao, Shuli,Chi, Yujie,Yi, Hongwei. 2014

[8]A novel laccase with inhibitory activity towards HIV-I reverse transcriptase and antiproliferative effects on tumor cells from the fermentation broth of mushroom Pleurotus cornucopiae. Wu, Xiangli,Huang, Chenyang,Chen, Qiang,Zhang, Jinxia,Wu, Xiangli,Wang, Hexiang,Wu, Xiangli,Wang, Hexiang.

[9]Expression, characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena. Bao, Wenhua,Peng, Rihe,Tian, Yongsheng,Zhao, Wei,Xue, Yong,Gao, Jianjie,Yao, Quanhong,Bao, Wenhua,Zhang, Zhen,Gao, Jianjie,Yao, Quanhong.

[10]ExpressionoflaccasegenefromwhiterotfungusinPichiapastoriscanenhancetheresistanceofyeasttoH-2O_2-mediatedoxidativestressbystimulatingthegIutathione-basedantioxidativesystem. 杨洋,范芳芳,卓睿,马富英,江木兰,张晓昱. 2012

[11]Heterologous expression and characterization of a laccase from Laccaria bicolor in Pichia pastoris. Wang, Bo,Wang, Xiuying,Tian, Yongsheng,Li, Zhenjun,Gao, Jianjie,Peng, Rihe,Yao, Quanhong,Wang, Xiuying,Yan, Ying. 2016

[12]A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea. Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Zhang, R. Y.,Ng, T. B.. 2011

[13]Characterization of a yeast recombinant laccase rLAC-EN3-1 and its application in decolorizing synthetic dye with the coexistence of metal ions and organic solvents. Zhuo, Rui,He, Feng,Zhang, Xiaoyu,Yang, Yang,Zhuo, Rui,Yang, Yang.

[14]Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes. Lin, Yaqiu,Zhang, Zhen,Xu, Zhisheng,Yao, Quanhong,Lin, Yaqiu,Tian, Yongsheng,Zhao, Wei,Zhu, Bo,Peng, Rihe,Yao, Quanhong.

[15]Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris. Sun, Jing,Xu, Hu,Liu, Da-Tong,Chen, Jian-Min,Sun, Jing,Peng, Ri-He,Xiong, Ai-Sheng,Tian, Yongsheng,Zhao, Wei,Yao, Quan-Hong.

[16]Heterologous expression and characterization of laccase 2 from Coprinopsis cinerea capable of decolourizing different recalcitrant dyes. Tian, Yong-Sheng,Xu, Hu,Peng, Ri-He,Yao, Quan-Hong,Wang, Rong-Tan.

[17]Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus. Zhang, Jinjing,Chen, Hui,Chen, Mingjie,Huang, Jianchun,Wang, Hong,Feng, Zhiyong,Zhang, Jinjing,Chen, Hui,Chen, Mingjie,Huang, Jianchun,Wang, Hong,Feng, Zhiyong,Ren, Ang,Zhao, Mingwen,Feng, Zhiyong.

[18]The laccase gene (LAC1) is essential for Colletotrichum gloeosporioides development and virulence on mango leaves and fruits. Wei, Yunxie,Liu, Yanan,Zhou, Fangxue,Zhang, Kaili,Liu, Xiaomei,Wei, Yunxie,Liu, Yanan,Zhou, Fangxue,Zhang, Kaili,Liu, Xiaomei,Pu, Jinji,Zhang, He. 2017

[19]The synergistic effect on production of lignin-modifying enzymes through submerged co-cultivation of Phlebia radiata, Dichomitus squalens and Ceriporiopsis subvermispora using agricultural residues. Dong, Ya-Chen,Fu, Ming-Liang,Chen, Qi-He,Wang, Wei,Hu, Zhong-Ce.

[20]Isolation of laccase gene from Bacillus subtilis and analysis of its physicochemical properties. Yang, Sui-Shan,Liu, Zhen-Wang,Yi, Xiao-Ping,Zhang, Ai-Lian,Zhang, Ze-Hua,Shen, Jin-Cheng,Yin, Hui-Xiang,Chen, Li-Ping,Zhang, Tian-Yuan,Luo, Jin-Xian,Zhang, Tian-Yuan,Luo, Jin-Xian.

作者其他论文 更多>>

微信小程序