Identification of a novel broad-spectrum anti-fungal strain of Pseudomonas aeruginosa (SU8) and effect of its crude metabolites against Rhizoctonia solani and Pyricularia oryzae

文献类型： 外文期刊

第一作者： Liao, X.

作者： Liao, X.;Su, P.;Wang, C.

作者机构：

关键词： Pseudornonas aeruginosa;Biocontrol;Rhizoctonia solani;Pyricularia oryzae

期刊名称：EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL （ 影响因子：1.995； 五年影响因子：1.115 ）

ISSN： 1110-1768

年卷期： 2015 年 25 卷 2 期

页码：

收录情况： SCI

摘要： The objective of this study was to evaluate the potential applications of the antagonistic bacterium SU8 for biocontrol of plant diseases. In this study, the bacterial stain SU8 showed high anti-fungal activity and characterized using morphological, physiological, biochemical tests, sequencing of 16S rDNA genes, and phylogenetic analysis. Its crude metabolites as ferment filtrate was evaluated for its inhibitory effect on the pathogenicity of Rhizoctonia solani and Pyricularia oryzae on rice, and several verification experiments were conducted to evaluate its efficacy for controlling infection. The antagonistic bacteria SU8 was identified to be Pseudomonas aeruginosa and was registered in GenBank with a record number of HQ283487. Immersion treatment of Rhizoctonia solani and Pyricularia oryzae with SU8 crude metabolites in fermented filtrate demonstrated a superior pathogenicity-reducing effect on the pathogens infected rice, as demonstrated by a reduction in the plaque size on leaves infected with Rhizoctonia solani and Pyricularia oryzae by 0.4%-63.6%, 0.5%-58.3%, respectively. Furthermore, the lesion sizes were further reduced with increased soaking time and higher concentrations of the fermented filtrate. Spore germination of P. oryzae was inhibited (EC50 = 548.3457 mu g/mL), but its toxicity was lower than that of Tricyclazole. The fermented filtrate reduced both rice sheath blight and rice blast up to 70% in pot cultures of rice plants by the concentration 120 mu g/mL, showing better protection than that reported for other microbial biocontrol agents previously. The goals for utilizing SU8 were protection and therapy. Field experiment results showed that the SU8 fermented filtrate was able to effectively control rice sheath blight and rice blast, with reductions of 23.8-63.9% and 29.9-61.9%, respectively. However, the highest level of control achieved with the fermented filtrate was lower than that of Jinggangmycin and Tricyclazole. The antagonistic bacterial isolate SU8 showed superior potential as a bio-control agent in controlling rice sheath blight and rice blast. The high antifungal activity of its metabolites in fermented filtrate shows the possibility that it may be used to develop new fungicides.

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