Oxidative burst activity in haemocytes of the freshwater prawn Macrobrachium rosenbergii

文献类型： 外文期刊

第一作者： Xian, Jian-An

作者： Xian, Jian-An;Zhang, Xiu-Xia;Li, Jun-Tao;Wang, Dong-Mei;Wang, An-Li;Zheng, Pei-Hua;Lu, Yao-Peng;Ye, Jian-Min

作者机构：

关键词： Oxidative burst;Macrobrachium rosenbergii;Haemocyte;Flow cytometry;Reactive oxygen species

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 73 卷

页码：

收录情况： SCI

摘要： Oxidative burst, release of reactive oxygen species/reactive nitrogen species (ROS/RNS) contributed to microorganisms killing, is a vital immune response of crustacean haemocyte. Three morphologic haemocyte types (hyaline cells, HC; semigranular cells, SGC; granular cells, GC) have been defined in crustaceans, and found to play different roles in immune defense. However, oxidative burst activities of different haemocyte subpopulations in crustaceans are currently not documented. In the present study, we investigated the oxidative burst activities of the three haemocyte types in the freshwater prawn Macrobrachium rosenbergii using the common ROS fluorescent probe dichlorofluorescin-diacetate (DCFH-DA). Nitric oxide (NO) donor sodium nitroprusside (SNP) improved the DCF fluorescence in haemocytes, while NO scavenger C-PTIO (2-(4-carboxypheny1)-4,4,5,5tetramethylimidazoline-1-oxy1-3-oxide) and NO-synthase inhibitor N-monomethyl-L-arginine (L-NMMA) reduced the fluorescence, suggesting that DCF fluorescence intensity could also be modified by intracellular NO level and activity of NO-synthase pathway. ROS/RNS was also produced in the untreated haemocytes. GC contained most non-induced ROS/RNS production, while oxidative activity of HC was rather weak. No significant impact of PMA could be observed on ROS/RNS level in all the three cell types. Both zymosan A (ZA) and lipopolysaccharide (LPS) significantly triggered the production of ROS/RNS in SGC and GC, whereas they had no effect on those of HC, suggesting that SGC and GC were the primary cell types involved in pathogens killing by ROS/RNS pathway. Cytochalasin B (Cyt B) inhibited the ZA-induced ROS/RNS production, but could not change the ROS/RNS level stimulated by LPS. For unstimulated haemocytes, ROS/RNS productions decreased 29.6%, 44.1% and 48.6% in SGC, and decreased 44.5%, 28.4% and 57.3% in GC, in the presence of L-NMMA, Fccp and DPI respectively, whereas apocynin could not modulate DCF fluorescence in both SGC and GC, suggesting that mitochondrial oxidative pathway was relatively more dominant in SGC, and NO-synthase (NOS) pathway appeared more active in GC. For LPS-stimulated haemocytes, oxidative activities decreased 22.9%, 42.9%, 29.6% and 60.0% in SGC, and reduced 40.6%, 25.2%, 26.7% and 70.6% in GC with the presence of L-NMMA, apocynin, Fccp and DPI respectively, suggesting that NADPH-oxidase (NOX) pathway in both SGC and GC was activated by LPS, and it became the predominant oxidative pathway in stimulated SGC, while NOS pathway was the relative main source for ROS/RNS production in stimulated GC.

分类号：

  • 相关文献

[1]Cellular responses of the tiger shrimp Penaeus monodon haemocytes after lipopolysaccharide injection. Xian, Jian-An,Zhang, Xiu-Xia,Wang, Dong-Mei,Xian, Jian-An,Wang, An-Li,Guo, Hui.

[2]Various cellular responses of different shrimp haemocyte subpopulations to lipopolysaccharide stimulation. Xian, Jian-An,Zhang, Xiu-Xia,Wang, Dong-Mei,Li, Jun Tao,Zheng, Pei-Hua,Lu, Yao-Peng.

[3]Haemocyte protein expression profiling of scallop Chlamys farreri response to acute viral necrosis virus (AVNV) infection. Wang, Chongming,Chen, Guofu,Zhang, Chunyun,Xu, Zhong,Yan, Peisheng,Li, Chenghua.

[4]In Vivo Monitor Oxidative Burst Induced by Cd2+ Stress for the Oilseed Rape (Brassica napus L.) Based on Electrochemical Microbiosensor. Xu, Qiao,Wang, Zhan,Yang, Qin,Zhao, Yuan-Di,Wei, Fang,Chen, Hong. 2010

[5]In vivo monitoring of oxidative burst induced by ultraviolet A and C stress for oilseed rape by microbiosensor. Xu, Qiao,Wang, Zhan,Yang, Qin,Zhao, Yuan-Di,Wei, Fang,Chen, Hong.

[6]Microsensor in vivo monitoring of oxidative burst in oilseed rape (Brassica napus L.) leaves infected by Sclerotinia sclerotiorum. Xu, Qiao,Liu, Sheng-Yi,Guo, Xue-Lan,Dong, Xu-Yan,Li, Pei-Wu,Chen, Hong,Xu, Qiao,Zou, Qiu-Ju,Zhao, Yuan-Di,Xu, Qiao,Zou, Qiu-Ju,Zhao, Yuan-Di,Song, Dan-Yang,Chen, Hong.

[7]Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus). Dong, Xiangbai,Ji, Ruiqin,Guo, Xuelan,Dong, Caihua,Liu, Yueying,Liu, Shengyi,Dong, Xiangbai,Ji, Ruiqin,Guo, Xuelan,Dong, Caihua,Liu, Yueying,Liu, Shengyi,Dong, Xiangbai,Foster, Simon J.,Chen, Hong,Hu, Qiong.

[8]Elicitation of the hypersensitive responses in tabacco by a 10.6 kD proteinaceous elicitor from Phytophthora palmi. Cai, YY,Chen, J. 1999

[9]Genetic parameters and response to selection for harvest body weight of the giant freshwater prawn Macrobrachium rosenbergii. Luan, Sheng,Luo, Kun,Kong, Jie,Yang, Guoliang,Wang, Junyi,Zhang, Yufei,Gao, Qiang,Yang, Guoliang,Wang, Junyi,Zhang, Yufei,Gao, Qiang,Hu, Honglang. 2012

[10]Current status of the giant freshwater prawn ( Macrobrachium rosenbergii) industry in China, with special reference to live transportation. Yang, Guoliang,Chen, Xuefeng,Wang, Junyi,Gao, Qiang,Frinsko, Mike,Hu, Guo. 2012

[11]Characterization of a novel nm23 gene and its potential roles in gametogenesis in the prawn Macrobrachium rosenbergii (de Man, 1879) (Crustacea: Decapoda). Song, Ya-Nan,Lu, Cui-Yun,Chen, Jie,Qiu, Gao-Feng,Lu, Cui-Yun.

[12]Effects of water-borne copper on the gills and hepatopancreas of Macrobrachium rosenbergii. Li, Na,Ma, Qiang,Zhao, Yunlong,Yang, Jian,Li, Na,Brochon, Jean-Claude. 2008

[13]Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii. Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Zhang, Zhao,Zhang, Chi-Yu,Li, Xin-chang. 2012

[14]Effects of anthraquinones extracted from Rheum officinale Bail on the growth, non-specific immune response of Macrobrachium rosenbergii. Liu, Bo,Ge, Xianping,Xie, Jun,Xu, Pao,Liu, Bo,Ge, Xianping,He, Yanhui,Xie, Jun,Xu, Pao,He, Yijin,Zhou, Qunlan,Pan, Liangkun,Chen, Ruli. 2010

[15]Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection. Ning, Mingxiao,Xiu, Yunji,Yuan, Meijun,Bi, Jingxiu,Liu, Min,Wei, Panpan,Yan, Yuye,Gu, Wei,Wang, Wen,Meng, Qingguo,Xiu, Yunji,Gu, Wei,Meng, Qingguo. 2017

[16]Genetic parameters of survival for six generations in the giant freshwater prawn Macrobrachium rosenbergii. Luan, Sheng,Luo, Kun,Kong, Jie,Wang, Junyi,Yang, Guoliang,Chen, Xuefeng,Gao, Qiang,Wang, Junyi,Yang, Guoliang,Chen, Xuefeng,Gao, Qiang,Hu, Honglang.

[17]iTRAQ-based quantitative proteomic analysis of Macrobrachium rosenbergii hemocytes during Spiroplasma eriocheiris infection. Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo,Xiu, Yunji,Liu, Xiaoqian,Hou, Libo,Xiu, Yunji,Wang, Jian,Liu, Yuhan,Gu, Wei,Wang, Wen,Meng, Qingguo.

[18]Expression Profile of Antioxidant Enzymes in Hemocytes from Freshwater Prawn Macrobrachium rosenbergii Exposed to an Elevated Level of Copper. Guo, Hui,Miao, Yu-Tao,Qian, Kun,Wang, An-Li,Guo, Hui,Xian, Jian-An.

[19]Effect of harvest time and soaking treatment on cell cycle activity in sugarbeet seeds. Sliwinska, E,Jing, HC,Job, C,Job, D,Bergervoet, JHW,Bino, RJ,Groot, SPC. 1999

[20]Characteristics of New Triploid Seedless Table Grape Cultivar 'Champion Seedless' Released in China. Zhao, S. J.,Zhang, X. Z.,Guo, Z. J.,Ma, A. H.. 2009

作者其他论文 更多>>

微信小程序