Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation
文献类型: 外文期刊
第一作者: Li, Rong
作者: Li, Rong;Xin, Shan;Tao, Chengcheng;Li, Hongbin;Jin, Xiang
作者机构:
关键词: Gossypium hirsutum;ascorbate oxidase;apoplast oxidation;cell elongation;auxin
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )
ISSN: 1422-0067
年卷期: 2017 年 18 卷 7 期
页码:
收录情况: SCI
摘要: Ascorbate oxidase (AO) plays an important role in cell growth through the modulation of reduction/oxidation (redox) control of the apoplast. Here, a cotton (Gossypium hirsutum) apoplastic ascorbate oxidase gene (GhAO1) was obtained from fast elongating fiber tissues. GhAO1 belongs to the multicopper oxidase (MCO) family and includes a signal peptide and several transmembrane regions. Analyses of quantitative real-time polymerase chain reaction (QRT-PCR) and enzyme activity showed that GhAO1 was expressed abundantly in 15-day post-anthesis (dpa)wild-type (WT) fibers in comparison with fuzzless-lintless (fl) mutant ovules. Subcellular distribution analysis in onion cells demonstrated that GhAO1 is localized in the cell wall. In transgenic tobacco bright yellow-2 (BY-2) cells with ectopic overexpression of GhAO1, the enhancement of cell growth with 1.52-fold increase in length versus controls was indicated, as well as the enrichment of both total ascorbate in whole-cells and dehydroascorbate acid (DHA) in apoplasts. In addition, promoted activities of AO and monodehydroascorbate reductase (MDAR) in apoplasts and dehydroascorbate reductase (DHAR) in whole-cells were displayed in transgenic tobacco BY-2 cells. Accumulation of H2O2, and influenced expressions of Ca2+ channel genes with the activation of NtMPK9 and NtCPK5 and the suppression of NtTPC1B were also demonstrated in transgenic tobacco BY-2 cells. Finally, significant induced expression of the tobacco NtAO gene in WT BY-2 cells under indole-3-acetic acid (IAA) treatment appeared; however, the sensitivity of the NtAO gene expression to IAA disappeared in transgenic BY-2 cells, revealing that the regulated expression of the AO gene is under the control of IAA. Taken together, these results provide evidence that GhAO1 plays an important role in fiber cell elongation and may promote cell growth by generating the oxidation of apoplasts, via the auxin-mediated signaling pathway.
分类号:
- 相关文献
作者其他论文 更多>>
-
The effect of [Br/Zn] content on the structure and photocatalytic performance of BiOBr/ZnO based composite coatings on NiO/Ni foam
作者:Li, Dayu;Li, Longfei;Liu, Yuxue;Lin, Hui;Wang, Yichen;Zhang, Yongcai;Wang, Yichen;Li, Hongbin;Hou, Zhenhua;Asghar, Zeeshan;Hou, Jianhua
关键词:BiOBr/ZnO composite; Solvothermal synthesis method; Nickel foam; Coating
-
Anti-infective properties of mung bean ( Vigna radiata (L.)R. L .)R. Wilczek) coat extract on Pseudomonas aeruginosa-infected-infected Caenorhabditis elegans: Transcriptomics and pathway analysis
作者:Tao, Mingfang;Peng, Xitian;Liao, Xianqing;Xia, Zhenzhen;Zheng, Dan;Li, Rong;Tao, Mingfang;Huang, Yuting;Xu, Tingting;Xu, Xiaoyun
关键词:Mung bean coat extract; C. elegans; Pseudomonas aeruginosa 14; Transcriptome; SKN-1; MAPK; Aak-2; Sir-2.1; Daf-2
-
Refined highland barley ameliorates obesity-associated insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and liver transcriptomics
作者:Wu, Tong;Dai, Zijian;Luo, Yingting;Yu, Qinye;Zhang, Yiyun;Bao, Xin;Li, Rong;Shen, Qun;Xue, Yong;Wu, Tong;Dai, Zijian;Zhang, Yiyun;Bao, Xin;Li, Rong;Shen, Qun;Xue, Yong;Zhang, Yuhong;Hao, Jing;Hao, Jing
关键词:Refined highland barley; Inflammation; Hypoglycemic effect; Gut microbiota; Liver transcriptome
-
Widespread impact of transposable elements on the evolution of post-transcriptional regulation in the cotton genus Gossypium
作者:Tian, Xuehan;Wang, Ruipeng;Liu, Zhenping;Lu, Sifan;Chen, Xinyuan;Zhang, Zeyu;Zhang, Xianlong;Wang, Maojun;Zhang, Zeyu;Liu, Fang;Li, Hongbin;Wang, Maojun
关键词:
Gossypium ; Transposable element; Direct RNA sequencing; Alternative splicing; Translation; MiRNA -
Transcriptomic and proteomic studies of body size and carcass traits and the longest dorsal muscle in Tibetan sheep
作者:Liu, Dehui;Li, Xue;Han, Buying;Wang, Song;Tian, Fei;Liu, Sijia;Zhao, Kai;Tian, Dehong;Liu, Dehui;Wang, Song;Wang, Lei;Pei, Quanbang;Zhao, Jincai;Ren, Qianben;Zhang, Wenkui;Liu, Dehui;Jiang, Hanjing;Sun, De;Li, Rong;Bao, Guoxiang
关键词:Body size traits; Carcass traits; Proteomics; Tibetan sheep; Transcriptomics; WGCNA
-
Cessation of manure application diminishes the dissemination potential of antibiotic resistance genes by altering bacterial interaction patterns in soil-lettuce systems
作者:Li, Ruochen;Pei, Xin;Zhang, Ming;Deng, Xuhui;Tao, Chengyuan;Wang, Jiabao;Shen, Zongzhuan;Li, Rong;Shen, Qirong;Chen, Xueli;Clarke, Nicholas;Sas-Paszt, Lidia
关键词:Antimicrobial resistance; Soil-lettuce systems; Poultry manure; Microbial network interaction; Source tracking; High-throughput qPCR
-
Genetic Diversity Analysis and Construction of a Core Germplasm Resource Bank of Xinjiang's Indigenous Cultivated Grapes
作者:Liu, Shiqing;Li, Hongbin;Shi, Wei;Liu, Shiqing;Wang, Jiancheng;Li, Xuerong;Wang, Xiyong;Shi, Wei;Wang, Jiancheng;Wang, Xiyong;Shi, Wei;Wang, Jiancheng;Wang, Xiyong;Shi, Wei;Wang, Xianhang;Zhong, Haixia;Aibibul, Ilham;Sun, Feng
关键词:Thompson Seedless; Munake grapes; whole-genome resequencing; genetic diversity; SSR; SNP; germplasm resource safety; intensive cultivation