Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response

文献类型： 外文期刊

第一作者： Liu, Linya

作者： Liu, Linya;Huang, Yacheng;Wu, Wenqiang;Xu, Yun;Cong, Ziwen;Xie, Jun;Xia, Wei;Huang, Dongyi;Liu, Linya;Huang, Yacheng;Huang, Xiaolong;Yang, Jianghua

作者机构：

关键词： dioscorin;cloning and expression;tuber development;promoter;Dioscorea alata L

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 7 期

页码：

收录情况： SCI

摘要： Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from Dioscorea alata L., comprising three class A (Da-dio1, -3 and -4) and two class B (Da-dio2 and -5) isoforms. Expressions of all dioscorin genes gradually decreased in mother tubers during yam sprouting and regrowth. On the other hand, all dioscorin genes accumulated transcripts progressively with tuber development in new tubers, with Da-dio5 being the most prominent isoform. In yam leaves, the expressions of Da-dio5 were up-regulated by the treatments of five phytohormones (gibberellic acid, salicylic acid, indole-3-acetic acid, abscisic acid, and ethylene), and three abiotic stresses (high-temperature, low-temperature and drought). To further elucidate the regulatory mechanisms of Da-dio5 expressions, transgenic Arabidopsis plants harboring the Da-dio5 promoter-beta-glucuronidase (GUS) fusion were generated. GUS staining showed that expressions of the Da-dio5 promoter were detected mainly in the shoot apical meristem (SAM) and hypocotyls, and enhanced by the treatments of the five hormones, and the three abiotic stresses mentioned above. These results suggest diverse roles of Da-dio5 in yam sprouting, regrowth, and tuberization, as well as in response to enviromental cues.

分类号：

  • 相关文献

[1]Phytohormone Profiling During Tuber Development of Chinese Yam by Ultra-high performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry. Luo, Hailing,Wang, Aiqin,Zhou, Yunyi,Huang, Wenjing,He, Longfei,Gong, Mingxia,Luo, Hailing,Gong, Mingxia,Luo, Hailing,Zhu, Pingchuan,He, Longfei.

[2]Transciptome analysis reveals flavonoid biosynthesis regulation and simple sequence repeats in yam (Dioscorea alata L.) tubers. Wu, Zhi-Gang,Jiang, Wu,Bao, Xiao-Qing,Chen, Song-Lin,Tao, Zheng-Ming,Bao, Xiao-Qing,Chen, Song-Lin,Mantri, Nitin. 2015

[3]Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake. Zhu, Derui,Liu, Jian,Liu, Deli,Zhu, Derui,Shen, Guoping,Long, Qifu,Wei, Xiaoxing,Han, Rui. 2014

[4]Cloning and expression of a 5-enolpyruvylshikimate-3-phosphate synthase gene from Halomonas variabilis. Liu, Zhu,Pan, Zhiwei,Xu, Yuquan,Dong, Zhiyang,Yang, Zhirong,Lin, Min.

[5]Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties. Miao, Jun,Sun, Jinghan,Liu, Dongcheng,Li, Bin,Zhang, Aimin,Li, Zhensheng,Tong, Yiping,Miao, Jun. 2009

[6]Regulatory mutations in the A2M gene are involved in the mastitis susceptibility in dairy cows. Wang, X. G.,Huang, J. M.,Feng, M. Y.,Ju, Z. H.,Wang, C. F.,Zhong, J. F.,Yang, G. W.,Yuan, J. D.. 2014

[7]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[8]Function identification of bovine Nramp1 promoter and intron 1. Hao, Linlin,Zhang, Libo,Liu, Songcai,Li, Mingtang,Zhong, Jifeng,Wang, Nan. 2011

[9]Identification and genetic effect of haplotypes in the promoter region of porcine myostatin gene. Liu, D.,Xu, Q.,Zang, L.,Liang, S.,Jiang, Y.,Wu, Y.,Wei, S.. 2011

[10]Specific Expression of Maize SBEIIb Promoter Mediated by Different Promoter Region in Transgenic Tobacco Plants. Sun Cui-xia,Li Meng,Wang Xiao-peng,Zhang Guo-dong,Tian Yan-chen,Wang Ze-li,Han Jing. 2009

[11]Identification and preliminary analysis of a new PCP promoter from Brassica rapa ssp chinensis. Zhang, Qiang,Cao, Jiashu,Zhang, Qiang,Liu, Huizhi. 2008

[12]p53 and NF kappa B regulate microRNA-34c expression in porcine ovarian granulosa cells. Xu Yuan,Xiao Guang,Zhang Zhe,Chen Zan-mou,Zhang Hao,Li Jia-qi,Zhang Ai-ling. 2016

[13]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[14]Promoter Characterization and Expression Pattern Analysis of Porcine TCAP Gene. Qiao, Mu,Wu, Huayu,Huang, Jingshu,Peng, Xianwen,Liu, Guisheng,Feng, Zheng,Mei, Shuqi. 2012

[15]The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns. Feng, Kun,Zheng, Qingsong,Feng, Kun,Yu, Jiahong,Cheng, Yuan,Ruan, Meiying,Wang, Rongqing,Ye, Qingjing,Zhou, Guozhi,Li, Zhimiao,Yao, Zhuping,Yang, Yuejian,Wan, Hongjian,Yu, Jiahong. 2016

[16]Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants. Zheng, Yusheng,Wang, Zhekui,Li, Dongdong,Xu, Li,Zhou, Peng,Ye, Rongjian,Lin, Yongjun,Ye, Rongjian,Lin, Yongjun. 2010

[17]Functional analysis of the larval serum protein gene promoter from silkworm, Bombyx mori.. Tang, SM,Yi, YZ,Shen, XJ,Zhang, ZF,Li, YR,He, JL. 2003

[18]Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters. Li, Jiaqi,Wang, Liangliang,Zhang, Hao,Chen, Songling,Mei, Yingjie,Wang, Chong,Ling, Fei,Du, HongLi,Fang, Wei,Chen, Yaosheng,Liu, Xiaohui. 2010

[19]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[20]Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo. Tang, SM,Zhao, QL,Yi, YZ,Zhang, ZF,Li, YR. 2005

作者其他论文 更多>>

微信小程序