Involvement of Chlamydomonas DNA damage tolerence gene UBC2 in lipid accumulation

文献类型： 外文期刊

第一作者： Li, Xinhan

作者： Li, Xinhan;Li, Ping;Deng, Xiaodong;Fei, Xiaowen

作者机构：

关键词： CrUBC2;Ubiquitin conjugating enzyme;Lys-63 polyubiquitination;DNA damage tolerance;Lipid accumulation;Chlamydomonas reinhardtii

期刊名称：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS （ 影响因子：4.401； 五年影响因子：5.278 ）

ISSN： 2211-9264

年卷期： 2017 年 22 卷

页码：

收录情况： SCI

摘要： The Lys-63-linked polyubiquitination of target proteins is considered a fundamentally different process from conventional Lys-48-linked polyubiquitination. This process is deemed analogous to other post-translational regulatory processes, such as phosphorylation and sumoylation, which alter target protein activities and are reported to be involved in several cellular processes. In this study, we report that CrUBC2, a yeast and Arabidopsis MMS2/UEV homolog gene in Chlamydomonas reinhardtii, is able to functionally rescue yeast mms2 mutant, leading to increased DNA damage tolerance. The overexpression of the CrUBC2 improves the algal ability to tolerate DNA damage, while silencing CrUBC2 sensitizes cells to DNA damage, indicating that CrUBC2 is involved in DNAdamage response in Chlamydomonas. Moreover, the overexpression of CrUBC2 increases algal cell lipid, while the silencing of CrUBC2 gene decreases lipid content, implying that a protein substrate of CrUBC13-CrUBC2 polyubiquitination is involved in lipid accumulation. (C) 2016 Elsevier B. V. All rights reserved.

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