Characterization of mutations in streptomycin-resistant Mycobacterium tuberculosis isolates in Sichuan, China and the association between Beijing-lineage and dual-mutation in gidB

文献类型： 外文期刊

第一作者： Sun, Honghu

作者： Sun, Honghu;Xiang, Ling;Pi, Rui;Guo, Zhen;Zheng, Chao;Li, Song;Sun, Qun;Zhang, Congcong;Zhao, Yuding;Tang, Ke;Luo, Mei;Rastogi, Nalin;Li, Yuqing

作者机构：

关键词： Mycobacterium tuberculosis;Streptomycin resistance;Dual-mutation;Beijing lineage

期刊名称：TUBERCULOSIS （ 影响因子：3.131； 五年影响因子：2.966 ）

ISSN： 1472-9792

年卷期： 2016 年 96 卷

页码：

收录情况： SCI

摘要： Mutations in rpsL, rrs, and gidB are well linked to streptomycin (STR) resistance, some of which are suggested to be potentially associated with Mycobacterium tuberculosis genotypic lineages in certain geographic regions. In this study, we aimed to investigate the mutation characteristics of streptomycin resistance and the relationship between the polymorphism of drug-resistant genes and the lineage of M. tuberculosis isolates in Sichuan, China. A total of 227 M. tuberculosis clinical isolates, including 180 STR-resistant and 47 pan-susceptible isolates, were analyzed for presence of mutations in the rpsL, rrs and gidB loci. Mutation K43R in rpsL was strongly associated with high-level streptomycin resistance (P < 0.01), while mutations in rrs and gidB potentially contributed to low-level resistance (P < 0.05). No general association was exhibited between STR resistance and Beijing genotype, however, in STR-resistant strains, Beijing genotype was significantly correlated with high-level STR resistance, as well as the rpsL mutation K43R (P < 0.01), indicating that Beijing genotype has an evolutionary advantage under streptomycin pressure. Notably, in all isolates of Beijing genotype, a dual mutation E92D (a276c) and A205A (a615g) in gidB was detected, suggesting a highly significant association between this dual mutation and Beijing genotype. (C) 2015 Elsevier Ltd. All rights reserved.

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