De Novo Assembly and Characterization of Fruit Transcriptome in Black Pepper (Piper nigrum)

文献类型： 外文期刊

第一作者： Hu, Lisong

作者： Hu, Lisong;Hao, Chaoyun;Fan, Rui;Wu, Baoduo;Tan, Lehe;Wu, Huasong;Hu, Lisong;Hao, Chaoyun;Tan, Lehe;Wu, Huasong;Fan, Rui;Wu, Baoduo;Tan, Lehe;Wu, Huasong

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Black pepper is one of the most popular and oldest spices in the world and valued for its pungent constituent alkaloids. Pinerine is the main bioactive compound in pepper alkaloids, which perform unique physiological functions. However, the mechanisms of piperine synthesis are poorly understood. This study is the first to describe the fruit transcriptome of black pepper by sequencing on Illumina HiSeq 2000 platform. A total of 56,281,710 raw reads were obtained and assembled. From these raw reads, 44,061 unigenes with an average length of 1,345 nt were generated. During functional annotation, 40,537 unigenes were annotated in Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes pathways, Swiss-Prot database, and Nucleotide Collection (NR/NT) database. In addition, 8,196 simple sequence repeats (SSRs) were detected. In a detailed analysis of the transcriptome, housekeeping genes for quantitative polymerase chain reaction internal control, polymorphic SSRs, and lysine/ornithine metabolism-related genes were identified. These results validated the availability of our database. Our study could provide useful data for further research on piperine synthesis in black pepper.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序