Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz)

文献类型： 外文期刊

第一作者： Li, Ruimei

作者： Li, Ruimei;Fan, Jie;Yang, Chenglong;Yao, Yuan;Zhou, Yang;Duan, Ruijun;Liu, Jiao;Fu, Shaoping;Guo, Jianchun;Li, Ruimei;Fan, Jie;Yang, Chenglong;Yao, Yuan;Zhou, Yang;Duan, Ruijun;Liu, Jiao;Fu, Shaoping;Guo, Jianchun;Ji, Yimeng;Fan, Jie;Yang, Chenglong;Yao, Yuan;Hu, Xinwen

作者机构：

关键词： Cassava;C-repeat binding factor;Cold tolerance;Gene expression;Low temperature

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN： 0137-5881

年卷期： 2014 年 36 卷 11 期

页码：

收录情况： SCI

摘要： Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4 A degrees C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava's high sensitivity to low temperature.

分类号：

  • 相关文献

[1]Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses. Li, Chuan,Cao, Moju,Li, Chuan,Rudi, Heidi,Fjellheim, Siri,Rognli, Odd Arne,Sandve, Simen R.,Stockinger, Eric J.,Cheng, Hongmei,Fox, Samuel E.,Fox, Samuel E.,Mockler, Todd C.,Westereng, Bjorge. 2012

[2]Screening and identification of microsatellite markers associated with cold tolerance in Nile tilapia Oreochromis niloticus. Zhu, H. P.,Liu, Z. G.,Lu, M. X.,Gao, F. Y.,Ke, X. L.,Huang, Z. H.. 2015

[3]Natural variation reveals that OsSAP16 controls low-temperature germination in rice. Wang, Xiang,Zou, Baohong,Shao, Qiaolin,Cui, Yongmei,Lu, Shan,Zhang, Yan,Huang, Ji,Hua, Jian,Huang, Quansheng,Hua, Jian. 2018

[4]Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress. Cho, Chang-Woo,Lee, Hye-Jeong,Chung, Eunsook,Kim, Kyoung Mi,Kim, Jee Eun Heo Jung-In,Chung, Jongil,Ma, Youzhi,Fukui, Kiichi,Lee, Dae-Won,Kim, Doh-Hoon,Chung, Young-Soo,Lee, Jai-Heou. 2007

[5]Expression Analysis of Low Temperature Responsive Genes in Eupatorium Adenophorum Spreng Using cDNA-AFLP. Wang, Junying,Zhang, Haiwen,Huang, Rongfeng.

[6]Functional conservation analysis and expression modes of grape anthocyanin synthesis genes responsive to low temperature stress. Zhang, Cheng,Jia, Haifeng,Fang, Jinggui,Wang, Chen,Wu, Weimin,Wang, Xicheng.

[7]Potential role of reactive oxygen species and antioxidant genes in the regulation of peach fruit development and ripening. Huan, Chen,Jiang, Li,An, Xiujuan,Xu, Yin,Yu, Zhifang,Yu, Mingliang,Ma, Ruijuan.

[8]Structure, Expression, and Functional Analysis of the Hexokinase Gene Family in Cassava. Geng, Meng-Ting,Wang, Yun-Lin,Hu, Xin-Wen,Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Wu, Xiao-Hui,Sun, Chong. 2017

[9]Genome-Wide Identification and Expression Analysis of the WRKY Gene Family in Cassava. Wei, Yunxie,Shi, Haitao,Xia, Zhiqiang,Tie, Weiwei,Ding, Zehong,Yan, Yan,Wang, Wenquan,Hu, Wei,Li, Kaimian. 2016

[10]Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes. Hu, Wei,Xia, Zhiqiang,Yan, Yan,Ding, Zehong,Tie, Weiwei,Zou, Meiling,Wei, Yunxie,Lu, Cheng,Hou, Xiaowan,Wang, Wenquan,Peng, Ming,Wang, Lianzhe. 2015

[11]Isolation and Characterization of Ftsz Genes in Cassava. Geng, Meng-Ting,Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Min, Yi,Chen, Xia,Fan, Jie,Yuan, Shuai,Wang, Lei,Zhang, Fan,Shang, Lu,Wang, Yun-Lin,Hu, Xin-Wen,Sun, Chong. 2017

[12]Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz). Ou, Wenjun,Li, Kaimian,Mao, Xiang,Huang, Chao,Tie, Weiwei,Yan, Yan,Ding, Zehong,Wu, Chunlai,Xia, Zhiqiang,Wang, Wenquan,Li, Kaimian,Hu, Wei,Zhou, Shiyi. 2018

[13]Identification, Expression, and Functional Analysis of the Fructokinase Gene Family in Cassava. Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Wang, Yun-Lin,Chen, Xia,Shang, Lu,Lu, Xiao-Hua,Li, Zhan,Hu, Xin-Wen,Wu, Xiao-Hui,Sun, Chong. 2017

[14]Cloning of 9-cis-epoxycarotenoid dioxygenase gene (TaNCED1) from wheat and its heterologous expression in tobacco. Zhang, S. J.,Song, G. Q.,Li, Y. L.,Gao, J.,Liu, J. J.,Fan, Q. Q.,Huang, C. Y.,Sui, X. X.,Chu, X. S.,Guo, D.,Li, G. Y.. 2014

[15]Exogenous 6-benzylaminopurine confers tolerance to low temperature by amelioration of oxidative damage in eggplant (Solanum melongena L.) seedlings. Chen, Jianlin,Wu, Xuexia,Zhu, Zongwen,Xu, Shuang,Zha, Dingshi,Wu, Xuexia,Zhu, Zongwen,Xu, Shuang,Zha, Dingshi,Yao, Xinfeng. 2016

[16]Bioaugmentation for biomass production at low temperature using enriched psychroactive methanogenic consortia. Cheng, Lei,Deng, Yu,Qiu, Tianlei,Sun, Xiaohong,Wang, Xuming,Han, Meilin,Cheng, Lei,Deng, Yu. 2011

[17]Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans under low temperature. Yin, Fei,Sun, Peng,Gao, Quanxin,Wang, Jiteng. 2016

[18]Metabolic changes of peanut (Arachis hypogaea L.) buds in response to low temperature (LT). Wang, X. J.,Sun, D. L.,Bian, N. F.,Zhang, Z. M.,Wang, X.,Xu, Z. J.,Qi, Y. J.,Shen, Y..

[19]Impact of arbuscular mycorrhizal fungi (AMF) on cucumber growth and phosphorus uptake under cold stress. Ma, Jun,Zou, Zhirong,Ma, Jun,Li, Yansu,Yu, Xianchang,Yan, Yan,He, Chaoxing,Janouskova, Martina.

[20]ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants. Xu, Dong-Bei,Li, Xue-Yin,Chen, Yao-Feng,Gao, Shi-Qing,Ma, You-Zhi,Xu, Zhao-Shi,Li, Lian-Cheng,Chen, Ming,Gao, Shi-Qing,Zhao, Chang-Ping,Tang, Yi-Miao,Li, Xue-Yin.

作者其他论文 更多>>

微信小程序