Determination of ACCase Sensitivity and Gene Expression in Quizalofop-Ethyl-Resistant and -Susceptible Barnyardgrass (Echinochloa crus-galli) Biotypes

文献类型： 外文期刊

第一作者： Wang, Jinxin

作者： Wang, Jinxin;Wang, Jinxin

作者机构：

关键词： HPLC;real-time PCR

期刊名称：WEED SCIENCE （ 影响因子：2.713； 五年影响因子：2.868 ）

ISSN： 0043-1745

年卷期： 2013 年 61 卷 4 期

页码：

收录情况： SCI

摘要： Mechanisms of herbicide resistance were studied in a quizalofop ethyl-resistant barnyardgrass biotype. Acetyl-coenzyme A carboxylase (ACCase) sensitivity to quizalofop-p-ethyl was measured by high-performance liquid chromatography and the trend in ACCase gene expression over time was determined using real-time polymerase chain reaction The results showed that an insensitive ACCase was present in Geqiushan resistant plants (R), with a resistance index of 106. The basal ACCase activities in Geqiushan Rand Geqiushan susceptible plants (S) were similar, at 1.20 and 1.17 ng malonyl-CoA min(-1) mu g(-1) extract protein, respectively. Basal ACCase gene expression in Geqiushan R was similar to that in Geqiushan S. The relative expression of ACCase gene decreased after spraying quizalofop ethyl at 60 g ai ha(-1) in Geqiushan S, whereas it was almost not changed in Geqiushan R. From these results we concluded that plastid ACCase sensitivity change might be responsible for the resistance and gene overexpression does not play a role in this resistance.

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