Genetic transformation and regeneration of Hevea brasiliensis transgenic plant with GAI gene by microparticle bombardment

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Chen, Xiongting;Peng, Shiqing;Wu, Kunxin;Hong, Lei

作者机构：

关键词： Genetic transformation;Hevea brasiliensis;Microparticle bombardment;GAI gene

期刊名称：ROMANIAN BIOTECHNOLOGICAL LETTERS （ 影响因子：0.765； 五年影响因子：0.823 ）

ISSN： 1224-5984

年卷期： 2013 年 18 卷 1 期

页码：

收录情况： SCI

摘要： A typhoon is a major natural disaster in the cultivation areas of Hevea brasiliensis in China. In this study, we report a method for the transformation and regeneration of H. brasiliensis transgenic plants expressing the Arabidopsis gibberellic acid insensitive (GAI) gene to induce the dwarfing phenotype. A pBI121vector comprising CaMV35S promoter, kanamycin resistance gene (nptII), beta-glucuronidase (GUS) reporter gene, and GAI gene was used. The embryoid induction rate was 3.21% using a 40-day-old calli as target with 1100 psi helium pressure and 9 cm target distance. Kanamycin-selected calli and embryos were positive for GUS activity in histochemical assays. A transgenic line and 21 sub-plantlets were positive for GAI gene amplification using the polymerase chain reaction. The integration of the GAI gene was confirmed by Southern blot and showed a single copy insertion. These results illustrate the advantages of using microparticle bombardment for stable plant transformation in H. brasiliensis with possible applications in other plant species.

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