Metabolite profiles of rice cultivars containing bacterial blight-resistant genes are distinctive from susceptible rice

文献类型: 外文期刊

第一作者: Huang, Xin

作者: Huang, Xin;Zhu, Shuifang;Wu, Jiao;Yu, Haichuan;Wu, Jiao;Dai, Haofu;Mei, Wenli;Peng, Ming

作者机构:

关键词: metabolomics;rice;gas chromatography;mass spectrometry;genetically modified

期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )

ISSN: 1672-9145

年卷期: 2012 年 44 卷 8 期

页码:

收录情况: SCI

摘要: The metabolic changes of bacterial blight-resistant line C418/Xa23 generated by molecular marker-assisted selection (n 12), transgenic variety C418-Xa21 generated by using the Agrobacterium-mediated system (n 12), and progenitor cultivar C418 (n 12) were monitored using gas chromatography/mass spectrometry. The validation, discrimination, and establishment of correlative relationships between metabolite signals were performed by cluster analysis, principal component analysis, and partial least squares-discriminant analysis. Significant and unintended changes were observed in 154 components in C418/Xa23 and 48 components in C418-Xa21 compared with C418 (P 0.05, Fold change 2.0). The most significant decreases detected (P 0.001) in both C418/Xa23 and C418-Xa21 were in three amino acids: glycine, tyrosine, and alanine, and four identified metabolites: malic acid, ferulic acid, succinic acid, and glycerol. Linoleic acid was increased specifically in C418/Xa23 which was derived from traditional breeding. This line, possessing a distinctive metabolite profile as a positive control, shows more differences vs. the parental than the transgenic line. Only succinic acid that falls outside the boundaries of natural variability between the two non-transgenic varieties C418 and C418/Xa23 should be further investigated with respect to safety or nutritional impact.

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