Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse

文献类型： 外文期刊

第一作者： Huang, Fengying

作者： Huang, Fengying;Meng, Qiuping;Tan, Guanghong;Huang, Yonghao;Wang, Hua;Huang, Fengying;Huang, Fengying;Mei, Wenli;Dai, Haofu

作者机构：

关键词： 16S rRNA;Gene sequences analysis;Staphylococcus haemolyticus;Multidrug resistant

期刊名称：ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE （ 影响因子：1.226； 五年影响因子：2.285 ）

ISSN： 1995-7645

年卷期： 2011 年 4 卷 6 期

页码：

收录情况： SCI

摘要： Objective: To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away front a hospital. Methods: Phenotype of the isolate was investigated by conventional microbiological methods, including Gram-staining, colony morphology, tests for haemolysis, catalase, coagulase, and antimicrobial susceptibility test. The mecA and 16S rRNA genes were amplified by the polymerase chain reaction (PCR) and sequenced. The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by phylogenetic analysis and multiple sequence alignment. Results: The isolate in this study was a gram positive, coagulase negative, and catalase positive coccus. The isolate was resistant to oxacillin, methicillin, penicillin, ampicillin, cefazolin, ciprofloxacin erythromycin, et al. PCR results indicated that the isolate was mecA gene positive and its 16S rRNA was 1 465 bp. Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus, and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the GenBank. Conclusions: 16S rRNA gene sequencing is a suitable technique for non-specialist researchers. Laboratory animals are possible sources of lethal pathogens, and researchers must adapt protective measures when they manipulate animals.

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