Cloning and expression analysis of rubredoxin from cold-treated banana leaves
文献类型: 外文期刊
第一作者: Yuan, K. H.
作者: Yuan, K. H.;Cheng, P.;Zhang, L. L.;Qi, J. F.;Zhang, X. B.;Zhou, L. Y.;Zhang, Y. D.;Feng, R. J.;Lu, L. F.;Ren, Y.;Xu, X. L.
作者机构:
关键词: Rubredoxin;Expression;Banana;Stress response
期刊名称:PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:1.039; 五年影响因子:0.77 )
ISSN: 0031-9457
年卷期: 2010 年 79 卷
页码:
收录情况: SCI
摘要: A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of MaRd1 gene was mainly in leaves, pseudo-stems and immature fruits, while it was barely detectable in roots and flowers. Cold and salt stresses induced higher levels of MaRd1 transcript accumulation in leaves. This finding indicated a role of MaRd1 in the response to these abiotic stresses.
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