Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate

文献类型： 外文期刊

第一作者： Sun, Hai-Yan

作者： Sun, Hai-Yan;Peng, Ming;Wang, Lu;Liu, Jian-Wen

作者机构：

关键词： Aspergillus oryzae;Methyl glucoside sesqui-stearate;Raw starch degrading enzyme;Transcriptional level;Translational level

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2009 年 159 卷 1 期

页码：

收录情况： SCI

摘要： The effect of methyl glucoside sesqui-stearate (MGS) on the production of raw starch degrading enzyme (RSDE) by Aspergillus oryzae F-30 was studied in this paper. The activity of RSDE formed by Aspergillus oryzae F-30 was enhanced dramatically by the addition of MGS to the medium. As a result, with the addition of 1.5 g MGS in 1 L basal medium, RSDE activity and productivity were 107 U mL(-1) and 1.49 U mL(-1) h(-1), 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, respectively. The effect of MGS on the synthesis of RSDE by Aspergillus oryzae F-30 was also studied on a molecular level. It was observed that the regulation of RSDE synthesis in Aspergillus oryzae F-30 occurs at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of MGS at transcriptional level.

分类号：

  • 相关文献

[1]First record of Aspergillus oryzae (Eurotiales: Trichocomaceae) as an entomopathogenic fungus of the locust, Locusta migratoria (Orthoptera: Acrididae). You, Yinwei,Zhang, Long,You, Yinwei,Song, Yuan,Wang, Youzhi.

[2]Effects of koji-making with mixed strains on physicochemical and sensory properties of Chinese-type soy sauce. Chen, Zhi-Yao,Feng, Yun-Zi,Cui, Chun,Zhao, Hai-Feng,Zhao, Mou-Ming,Chen, Zhi-Yao.

[3]Expressing PHB synthetic genes through chloroplast genetic engineering. Zhang, JY,Su, N,Zhang, ZL,Zhao, HY,Zhu, SW,Song, YR. 2002

[4]Cloning and Expression of Two Soluble Acid Invertase Gene Isoforms from Rhododendron. He Lisi,Su Jiale,Liu Xiaoqing,Li Chang,Chen Shangping. 2014

作者其他论文 更多>>

微信小程序