Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate
文献类型: 外文期刊
第一作者: Sun, Hai-Yan
作者: Sun, Hai-Yan;Peng, Ming;Wang, Lu;Liu, Jian-Wen
作者机构:
关键词: Aspergillus oryzae;Methyl glucoside sesqui-stearate;Raw starch degrading enzyme;Transcriptional level;Translational level
期刊名称:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY ( 影响因子:2.926; 五年影响因子:2.685 )
ISSN: 0273-2289
年卷期: 2009 年 159 卷 1 期
页码:
收录情况: SCI
摘要: The effect of methyl glucoside sesqui-stearate (MGS) on the production of raw starch degrading enzyme (RSDE) by Aspergillus oryzae F-30 was studied in this paper. The activity of RSDE formed by Aspergillus oryzae F-30 was enhanced dramatically by the addition of MGS to the medium. As a result, with the addition of 1.5 g MGS in 1 L basal medium, RSDE activity and productivity were 107 U mL(-1) and 1.49 U mL(-1) h(-1), 4.3-fold and 7.1-fold greater than the values obtained in the basal medium, respectively. The effect of MGS on the synthesis of RSDE by Aspergillus oryzae F-30 was also studied on a molecular level. It was observed that the regulation of RSDE synthesis in Aspergillus oryzae F-30 occurs at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of MGS at transcriptional level.
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