Analysis of Primary Elemental Speciation Distribution in Mungbean During Enzymatic Hydrolization

文献类型： 外文期刊

第一作者： Li Ji-hua

作者： Li Ji-hua;Huang Mao-fang;Zhu De-ming;Zhong Ye-jun;Li Ji-hua;Huang Mao-fang;Li Ji-hua;Huang Mao-fang;Zhu De-ming;Zhong Ye-jun;Zheng Wei-wan

作者机构：

关键词： Trace element;Mungbean;Enzymatic hydrolization

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2009 年 29 卷 3 期

页码：

收录情况： SCI

摘要： In the present paper, trace elements contents of cuprum, zincum, manganese and ferrum in mungbean and their primary speciation distribution during enzymatic hydrolization were investigated with ICP-AES OPTIMA 5300DV plasma emission spectroscopy. The trace elements were separated into two forms, i.e. dissolvable form and particulate form, by cellulose membrane with 0.45 mu m of pore diameter. All the samples were digested by strong acid (perchloric acid and nitric acid with I : 4 ratio). The parameters of primary speciations of the four elements were calculated and discussed. The results showed: (1) Contents of cuprum, zincum, manganese and ferrum in mungbean were 12.77, 31.26, 18.14 and 69.38 mu g . g(-1) (of dry matter), respectively. Different treatment resulted in different elemental formulation in product, indicating that more attention should be paid to the trace elements pattern when producing mungbean beverage with different processes. (2) Extraction rates of cuprum, zincum, manganese and ferrum in extract were 68.84%, 51.84%, 63.97% and 30.40% with enzymatic treatments and 36.22%, 17.58%, 7.85% and 22.99% with boil treatment, respectively. Both boil and enzymatic treatments led to poor elemental extraction rates, which proved that it was necessary to take deep enzymatic hydrolysis treatment in mungbean beverage process as the trace element utilization rate was concerned. (3) Amylase, protease and cellulose showed different extraction effectiveness of the four trace elements. Generally, protease exhibited highest efficiency for the four elements extraction. All of the four trace elements were mostly in dissolvable form in all hydrolysates and soup. (4) Relative standard deviations and recovery yields are within 0.12%-0.90%(n=11) and 98.6%-101.4%, respectively. The analysis method in this paper proved to be accurate.

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