Inhibitory effects of hinokitiol on tyrosinase activity and melanin biosynthesis and its antimicrobial activities

文献类型: 外文期刊

第一作者: Zhu, Yu-Jing

作者: Zhu, Yu-Jing;Liu, Bo;Qiu, Ling;Zhou, Jing-Jing;Guo, Hua-Yun;Hu, Yong-Hua;Li, Zhi-Cong;Wang, Qin;Chen, Qing-Xi

作者机构:

关键词: Hinokitiol;tyrosinase;inhibition kinetics;melanin biosynthesis;antimicrobial activity

期刊名称:JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY ( 影响因子:5.051; 五年影响因子:4.598 )

ISSN: 1475-6366

年卷期: 2010 年 25 卷 6 期

页码:

收录情况: SCI

摘要: The inhibitory effects of hinokitiol, a constituent of the woody oils isolated from Cupressaceae heartwood, on mushroom tyrosinase and melanin formation in B16 melanoma cells as well as its antimicrobial activity were investigated. Our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. The IC(50) values were estimated as 9.67 mu M for monophenolase activity and 0.21 mu M for diphenolase activity. The lag time of the monophenolase activity was not obviously lengthened by the compound. Kinetic analyses showed that the inhibition mechanism of hinokitiol was a mixed-type inhibition of the diphenolase activity. Hinokitiol effectively inhibited both cellular tyrosinase activity and melanin biosynthesis in B16 melanoma cells with significant cytotoxicity. Furthermore, it was found that hinokitiol could inhibit the proliferation of Salmonella enteritidis, Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Klebsiella pneumoniae, and Ralstonia solanacearum to different extents. This research may widen the use of hinokitiol in the fields of food preservation, depigmentation, and insecticide use.

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