An Improved Isolation Method of Soy beta-Conglycinin Subunits and Their Characterization

文献类型： 外文期刊

第一作者： Yuan, De-Bao

作者： Yuan, De-Bao;Min, Wei;Yang, Xiao-Quan;Tang, Chuan-He;Huang, Ke-Li;Guo, Jian;Wang, Jin-Mei;Wu, Na-Na;Qi, Jun-Ru;Zheng, Heng-Guang

作者机构：

关键词： Subunits of beta-conglycinin;Isolation;Physicochemical characterization;Thermal aggregation;AFM

期刊名称：JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY （ 影响因子：1.849； 五年影响因子：2.299 ）

ISSN： 0003-021X

年卷期： 2010 年 87 卷 9 期

页码：

收录情况： SCI

摘要： This study aimed at establishing an effective preparative isolation method of soy beta-conglycinin constituent subunits and characterizing some of their physicochemical properties and their heat-induced aggregation. These subunits were isolated in relatively large amounts and in high purity by dissociating beta-conglycinin in 6 M urea and using a combination of DEAE-Sepharose fast flow column chromatography and immobilized metal ion affinity chromatography (IMAC). At a pH deviating from isoelectric point (pI), zeta potentials of alpha' and alpha subunits were much larger than that of beta subunit, while in the latter case, the hydrophobic groups were more buried within the proteins. Dynamic light scattering analysis indicated that the extent of heat-induced aggregation of beta subunit was much higher than that of alpha' and alpha subunits, and the aggregation was also more affected by the increase in ionic strength. Atomic force microscopy analysis indicated that more ordered and stranded aggregates were formed for alpha' and alpha subunits. These results confirm a close relationship between physicochemical properties and heat-induced aggregation of beta-conglycinin subunits.

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