DIVERSITY OF METHANE-PRODUCING MICROBIAL COMMUNITY STRUCTURE WITH 16S-V3 rDNA PCR-DGGE

文献类型： 外文期刊

第一作者： Na, Wang

作者： Na, Wang;Liu, Hui-Ying;Niu, Shi-Wei;Hong, Wang;Tao, Yang;Irfan, Muhammad

作者机构：

关键词： community structure;PCR-DGGE;uncultured bacteria

期刊名称：Genetika-Belgrade （ 影响因子：0.761； 五年影响因子：0.678 ）

ISSN： 0534-0012

年卷期： 2016 年 48 卷 3 期

页码：

收录情况： SCI

摘要： The method of 16S-V3 rDNA PCR-DGGE was utilized to study the diversity of methane-producing microbial community structure under different methane-producing microbial community structure . Results showed that community structure of the methanogens under different environmental condition was significantly different. The number of bands about community under extreme condition was less than normal conditions , but the main bands were obvious . The sequence analysis showed that the predominant strains mainly were uncultured bacteria that could not be identified in the level of species .Z2 was Clostridia , Z8 was Clostridiales , D5 was Eubacteriaceae , and D8 was Flavobacteriaceae . This study laid some extent foundation for future research about establishing a methane-producing bacterial system with high gas yield in winter.

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