DIVERSITY OF METHANE-PRODUCING MICROBIAL COMMUNITY STRUCTURE WITH 16S-V3 rDNA PCR-DGGE
文献类型: 外文期刊
第一作者: Na, Wang
作者: Na, Wang;Liu, Hui-Ying;Niu, Shi-Wei;Hong, Wang;Tao, Yang;Irfan, Muhammad
作者机构:
关键词: community structure;PCR-DGGE;uncultured bacteria
期刊名称:Genetika-Belgrade ( 影响因子:0.761; 五年影响因子:0.678 )
ISSN: 0534-0012
年卷期: 2016 年 48 卷 3 期
页码:
收录情况: SCI
摘要: The method of 16S-V3 rDNA PCR-DGGE was utilized to study the diversity of methane-producing microbial community structure under different methane-producing microbial community structure . Results showed that community structure of the methanogens under different environmental condition was significantly different. The number of bands about community under extreme condition was less than normal conditions , but the main bands were obvious . The sequence analysis showed that the predominant strains mainly were uncultured bacteria that could not be identified in the level of species .Z2 was Clostridia , Z8 was Clostridiales , D5 was Eubacteriaceae , and D8 was Flavobacteriaceae . This study laid some extent foundation for future research about establishing a methane-producing bacterial system with high gas yield in winter.
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