文献类型： 外文期刊
第一作者： Sun, Shao-kun
作者： Sun, Shao-kun;Yang, Ni-na;Chen, Li-jing;Irfan, Muhammad;Sun, Shao-kun;Chen, Li-jing;Li, Tian-lai;Zhao, Xing-hua
作者机构：
期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）
ISSN： 2314-6133
年卷期： 2015 年
页码：
收录情况： SCI
摘要： LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
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