Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

文献类型： 外文期刊

第一作者： Sun, Shao-kun

作者： Sun, Shao-kun;Yang, Ni-na;Chen, Li-jing;Irfan, Muhammad;Sun, Shao-kun;Chen, Li-jing;Li, Tian-lai;Zhao, Xing-hua

作者机构：

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2015 年

页码：

收录情况： SCI

摘要： LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.

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