Development of Head Smut Resistance-linked Sequence Characterized Amplified Regions Markers in Sorghum
文献类型: 外文期刊
第一作者: Li, Yueying
作者: Li, Yueying;Ma, Lianju;Ma, Chunyan;Li, Xuemei;Lu, Dan;Lu, Shuiyi;Hao, Lin;Zou, Jianqiu
作者机构:
关键词: Sorghum;Heead smut;Molecular marker;Random amplified polymorphic DNA;Sequence characterized amplified regions (SCAR)
期刊名称:INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY ( 影响因子:0.822; 五年影响因子:0.906 )
ISSN: 1560-8530
年卷期: 2012 年 14 卷 4 期
页码:
收录情况: SCI
摘要: Head smut caused by Sporisorium reilianum (Kuhn) Clinton is a major limiting factor to sorghum yield. To effectively screen or evaluate resistant varieties, we first developed sequence characterized amplified regions (SCAR) markers tightly linked to head smut physiological race 3 resistant trait in sorghum. Two primers (S18 & S336) of random amplified polymorphic DNA (RADP) were obtained from a total of 400 RAPD primers tested and used to amplify 144 sorghum F-2 progenies (96 resistant & 48 susceptible) from a cross between accessions Tx622B (susceptible) and 7050B (resistant), and polymorphic fragments of 799 bp and 1419 bp were produced, respectively. The linkage analyses between head smut resistance and the two RAPD markers showed that the recombinant rates for marker S18 and S336 were 8.33% and 10.42%, and corrensponding linkage map distances were 8.4 cM and 10.6 cM, respectively. Based on the DNA sequences of these two RAPD markers, two SCAR markers were developed and successfully tagged the resistant trait of sorghum head smut physiological race 3. This would provide a useful tool for the genetic improvement of sorghum resistance to head smut. (c) 2012 Friend Science Publishers
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