Somatic embryogenesis from mature sorghum seeds: An underutilized genome editing recipient system

文献类型： 外文期刊

第一作者： Wu, Han

作者： Wu, Han;Zhang, Kuangye;Li, Jia;Wang, Jiaxu;Wang, Yanqiu;Cong, Ling;Duan, Youhou;Ke, Fulai;Zhang, Fei;Liu, Zhiqiang;Lu, Feng;Zhang, Zhipeng;Zou, Jianqiu;Zhu, Kai;Yu, Junchi

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关键词： Somatic embryogenesis; Regeneration; Sorghum; Seeds; Genome editing; Recipient

期刊名称：HELIYON （ 影响因子：4.0； 五年影响因子：4.1 ）

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年卷期： 2024 年 10 卷 1 期

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收录情况： SCI

摘要： Somatic embryogenesis is a process of cell totipotency in vitro, whereby an embryogenic cell develops from vegetative tissues rather than from zygotes after double fertilization. Sorghum is a recalcitrant crop in genetic transformation; previous recipient systems have usually been from immature zygotic embryos, which needed more time and labors to prepare. Here, an efficient 2,4dichlorophenoxyacetic acid (2,4-D)-induced somatic embryogenesis system from mature sorghum seeds was introduced. 2,4-D can induce two types of calli from a plumular axis section. Lowconcentration 2,4-D (e.g., 2 mg/L) induces white and loose non-embryogenic calli (type 1), while high-concentration 2,4-D (e.g., 8 mg/L) induces yellow and compact embryogenic calli (type 2), which can be clearly distinguished by Sudan red staining. Germinating seeds have a long 2-day window for SE induction. Somatic embryogenesis can be enhanced by HDAC inhibitor, trichostatin A (TSA), a histone deacetylase treatment, which shows more SE productivity and a bigger size. Importantly, this easily prepared protocol does not show obvious genotype dependency in sorghum hybrids. In this study, a high-concentration 2,4-D-induced SE system was established from mature sorghum seeds. This finding provides a technical option for the genome editing recipient in sorghum.

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