Establishment of Adventitious Bud Regeneration System from Bulb of Muscari Botryoides Mill
文献类型: 外文期刊
第一作者: Sun, Hongmei
作者: Sun, Hongmei;Zhang, Jiao;Song, Lina;Sun, Hongmei;Tao, Chengguang
作者机构:
关键词: Muscari botryoides Mill;tissue culture;callus;adventitious bud
期刊名称:3RD CONFERENCE ON KEY TECHNOLOGY OF HORTICULTURE, CKTH 2011
ISSN:
年卷期: 2011 年
页码:
收录情况: SCI
摘要: The bulblets of Muscari botryoides Mill were selected as explants to develop the system of incubation in vitro on MS medium. Different sterilizing duration, various hormone as well as sucrose concentrations were designed for observing changes of the explants. The results show that the suitable sterilizing method of explants is to sterilize the scales for 8 minutes with 0.1 % HgCl2 solution generating that the contamination rate is only 4 %. The optimum combination for callus induction is MS medium with the addition of 2 mg/L 6BA, 0.5 mg/L NAA and 5 % sucrose. Dark condition is conducive to callus induction. The optimal proliferation medium for adventitious buds is MS supplemented with 2.0 mg/L 6BA, 0.1 mg/L NAA and 3 % sucrose. The appropriate rooting medium is MS in combination with 0.3 mg/LNAA and 3.0 % sucrose. The survival rate of transplant is 95 % when the seedlings are transplanted into the matrix containing peat, vermiculite and soil with volume ratio 2:1:1:2.
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