Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Chen, Wenjing;Chen, Hongyu;Gong, Ming;Shao, Youran;Bao, Dapeng;Zou, Gen;Chen, Wenjing;Wang, Li

作者机构：

关键词： Mycoprotein; CRISPR/Cas9; Molecular design breeding; Endo; N -acetyl-beta-D-glucosaminidase; Cordyceps militaris

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 305 卷

页码：

收录情况： SCI

摘要： Fungi comprise approximately 2 % of the Earth's biomass; however, the human gastrointestinal tract has a limited capacity to digest fungal biomass. In this study, a novel endo-N-acetyl-beta-D-glucosaminidase, Endo CM, was characterized in the mushroom-forming fungus Cordyceps militaris, where it plays a role in maintaining the integrity of the fungal cell wall. Through gene editing, the Endo CM promoter was engineered to remove the binding site of the CmCreA carbon catabolite repressor, and the transformant was named CmT. After 12 h of treatment with simulated digestive fluids, the residual mycelial biomass of CmT was reduced to 50.00 +/- 1.57 %, compared with 69.47 +/- 0.97 % (p = 0.00005) for the parent strain. CmT also released more amino acids during the simulated digestion, suggesting that the expression level of Endo CM affects the accessibility of mycelial biomass to digestive enzymes. Additionally, CmT produced fruiting bodies with improved flavor but impaired appearance. This study highlights the production of alternative proteins with high digestibility and provides a sustainable approach for breeding mushrooms with improved digestibility and absorption properties.

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