KINETICS OF SOIL UREASE AFFECTED BY UREASE INHIBITORS AT CONTRASTING MOISTURE REGIMES
文献类型: 外文期刊
第一作者: Juan, Y. H.
作者: Juan, Y. H.;Chen, L. J.;Wu, Z. J.;Juan, Y. H.;Wang, R.
作者机构:
关键词: kinetic parameters;mixed inhibition;moisture regime;soil urease
期刊名称:REVISTA DE LA CIENCIA DEL SUELO Y NUTRICION VEGETAL
ISSN: 0717-635X
年卷期: 2009 年 9 卷 2 期
页码:
收录情况: SCI
摘要: With black soil (Pachic Udic Argiboroll) of Northeastern China as the test object, an incubation test was conducted to investigate the effects of urease inhibitors, hydroquinone (HQ), phenyl phosphorodiamidate (PPD) and N-(n-Butyl) thiophosphoric triamide (NBPT), on the kinetic characteristics of soil urease under normal moisture and waterlogged conditions, aimed to study the changes of catalytic potential of soil urease and the inhibition mechanisms. The results showed that test urease exhibited typical Michaelis-Menten kinetic behaviors, and all test inhibitors increased the K(m) and decreased the V(max) of soil urease, behaving as mixed inhibitors to soil urease. Under both normal and waterlogged conditions, compared with HQ, PPD and NBPT made K(m) increase and V(max) and V(max)/K(m) decrease more greatly, and the duration of these effects was longer (ca. 30 days vs. 10 days). Under water-logging, PPD made more increment of K(m) and more decrement of V(max) and V(max)/K(m) than NBPT, compared with that under normal soil moisture condition, suggesting that NBPT was more available under normal soil moisture condition, while PPD was promising under water-logging condition. To apply urease inhibitors and to control soil moisture condition could be a feasible way in increasing fertilizer N use efficiency affected by soil urease.
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