The fine transformation between the oxidized and reduced forms of interleukin-1 receptor antagonist

文献类型： 外文期刊

第一作者： Li, Shugang

作者： Li, Shugang;Wang, Linmei

作者机构：

关键词： HPLC;RHIL-1ra;MALDI-TOF mass spectroscopy;oxidized form;reduced form;transformation

期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）

ISSN： 1045-1056

年卷期： 2008 年 36 卷 1 期

页码：

收录情况： SCI

摘要： The active and pure recombinant forms of human interleukin-1 receptor antagonist (rHIL-1ra) were obtained from inclusion body expressed in Escherichia coli BL21 (DE3). However, the final purified protein was found to be in an unstable reduced form and easily converted to the other forms. Which form of the protein was involved and precisely how this occurred remained obscure. Therefore, we changed oxidizing and reducing conditions to delineate the subtle change between the reduced and oxidized states of rHIL-1ra based on high-pressure liquid chromatography (HPLC). The fine transformation between the two states was further verified through matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectroscopy. Meanwhile, which form might be beneficial to the protein was also investigated by testing its full bioactivity based upon methyl thiazolyl tetrazolium (MTT) method. (C) 2007 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

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