Map-based cloning and functional analysis of YGL8, which controls leaf colour in rice (Oryza sativa)

文献类型： 外文期刊

第一作者： Zhu, Xiaoyan

作者： Zhu, Xiaoyan;Guo, Shuang;Wang, Zhongwei;Du, Qing;Xing, Yadi;Zhang, Tianquan;Shen, Wenqiang;Sang, Xianchun;Ling, Yinghua;He, Guanghua;Guo, Shuang;Du, Qing

作者机构：

关键词： Rice (Oryza sativa);Yellow-green leaf 8 (ygl8);Map-based cloning;Functional analysis

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2016 年 16 卷

页码：

收录情况： SCI

摘要： Background: As the indispensable part of plant, leaf blade mainly functions as the production workshops where organic substance is produced by photosynthesis. Leaf colour mutation is a genetic phenomenon that has a high frequency and is easily identified. The mutations always exhibit negative impact on the development of plants in any of the different stages of growth. Up to now, numerous genes involved in leaf colour mutations have been cloned. Results: In this study, a yellow-green leaf mutant, yellow-green leaf 8 (ygl8), with stable genetic phenotype, has been screened out in the progeny of an excellent indica restorer line Jinhui 10 with seeds treated by EMS. The levels of Chl a, Chl b and total chlorophyll were significantly lower in ygl8 than those in the WT throughout the whole growth period, while no clear change was noted in the Chl a/b ratio. Transmission electron microscopy demonstrated that the lamellae were clearly intumescent and intricately stacked in ygl8. Furthermore, compared with those of the WT, the stomatal conductance, intercellular CO2 concentration, photosynthetic rate and transpiration rate of ylg8 were all significantly lower. Map-based cloning results showed that Loc_Os01g73450, encoding a chloroplast-targeted UMP kinase, corresponded to Ygl8 and played an important role in regulating leaf colour in rice (Oryza sativa). Complementation of ygl8 with the WT DNA sequence of Loc_Os01g73450 led to restoration of the normal phenotype, and transgenic RNA interference plants showed a yellow-green colour. Analysis of the spatial and temporal expression of Ygl8 indicated that it was highly expressed in leaf blades and weakly expressed in other tissues. qRT-PCR also showed that the expression levels of the major Photosystem I core subunits plastome-encoded PsaA, PsaB and PsbC were significantly reduced in ygl8. The expression levels of nuclear-encoded gene involved in Chl biosynthesis HEMC, HEME, and PORA were also decreased when compared with the wild-type. Conclusions: Independent of Chl biosynthesis and photosystem, YGL8 may affect the structure and function of chloroplasts grana lamellae by regulating plastid genome encoded thylakoid membrane constitutive gene expression and indirectly influences Chl biosynthesis.

分类号：

  • 相关文献

[1]Cloning, expression and functional analysis of a delta 6-desaturase gene from the silkworm, Bombyx mori L.. Yu, Hai-Yan,Zhou, Zhi-Feng,Jia, Jun-Qiang,Gui, Zhong-Zheng,Jia, Jun-Qiang,Gui, Zhong-Zheng. 2016

[2]Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans. Cheng, Jianhui,Xu, Xin,Wang, Liangyan,Tian, Bing,Hua, Yuejin,Wang, Hu. 2015

[3]Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives. Zhang, Ling-Ling,Zhong, Xiao-Juan,Jiang, Qian-Tao,Ma, Jian,Qi, Peng-Fei,Li, Wei,Chen, Guo-Yue,Lan, Xiu-Jin,Deng, Mei,Wei, Yu-Ming,Zheng, You-Liang,Wang, Qing,Lu, Zhen-Xiang,Pu, Zong-Jun. 2017

[4]Functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick Rhipicephalus haemaphysaloides. Zhang, Houshuang,Yang, Siqi,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin,Yang, Siqi.

[5]Characterization and functional analysis of a novel PCP gene BcMF5 from Chinese cabbage (Brassica campestris L. ssp chinensis Makino). Cao, Jiashu,Huang, Li,Xiang, Xun,Yu, Xiaolin,Zhang, Qiang,Liu, Huizhi. 2008

[6]A potential virulence factor involved in fungal pathogenicity: serine-like protease activity of nematophagous fungus Clonostachys rosea. Zhao, ML,Huang, JS,Mo, MH,Zhang, KQ. 2005

[7]Cgl2 plays an essential role in cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata). Liu, Dongming,Ye, Zhibiao,Liu, Dongming,Tang, Jun,Liu, Zezhou,Dong, Xin,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao,Sun, Peitian,Liu, Yumei,Li, Zhansheng,Fang, Zhiyuan,Yang, Limei. 2017

[8]Functional analyses of an E3 ligase gene AIP2 from wheat in Arabidopsis revealed its roles in seed germination and pre-harvest sprouting. Gao, Dong-Yao,Xu, Zhao-Shi,He, Yi,Sun, Yong-Wei,Ma, You-Zhi,Xia, Lan-Qin,Gao, Dong-Yao. 2014

[9]Functional Analysis and Marker Development of TaCRT-D Gene in Common Wheat (Triticum aestivum L.). Wang, Jiping,Li, Runzhi,Mao, Xinguo,Jing, Ruilian. 2017

[10]Transcript suppression of TaGW2 increased grain width and weight in bread wheat. Hong, Yantao,Chen, Longfei,Du, Li-pu,Wang, Jinfen,Ye, Xingguo,Qi, Lin,Zhang, Zengyan,Su, Zhenqi.

[11]Distinct expression pattern of miRNAs in Marek's disease virus infected-chicken splenic tumors and non-tumorous spleen tissues. Li, Zhi-jie,Zhang, Yan-ping,Zheng, Hui-wen,Zheng, Yong-sheng,Liu, Chang-jun,Li, Yang.

[12]Screening for S32G mutation of BMP15 gene in 18 goat breeds. Feng, Tao,Feng, Tao,Chu, Ming-Xing,Cao, Gui-Lin,Huang, Dong-Wei,Di, Ran,Liu, Qiu-Yue,Pan, Zhang-Yuan,Jin, Mei,Zhang, Ying-Jie. 2014

[13]miR-1338-5p Modulates Growth Hormone Secretion and Glucose Utilization by Regulating ghitm in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Qiang, Jun,Li, Hong Xia,He, Jie,Tao, Yi Fan,Xu, Pao,Bao, Jing Wen,Chen, De Ju. 2017

[14]Immunomodulatory roles and functional analysis of pre-B lymphocyte DT40 cells with the bursal-derived BSP-II treatment. Feng, Xiu-Li,Zhou, Bin,Cao, Rui-Bing,Liu, Ke,Liu, Xiao-Dong,Zhang, Yuan-Peng,Huang, Li,Ji, Xiang-Bo,Chen, Pu-Yan,Liu, Qing-Tao,Luo, Jun,Zhang, Gaiping.

[15]Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China. Katiyar, SK,Tan, Y,Huang, B,Chandel, G,Xu, Y,Zhang, Y,Xie, Z,Bennett, J. 2001

[16]Mapping quantitative trait loci associated with arsenic accumulation in rice (Oryza sativa). Zhang, Jing,Zhu, Yong-Guan,Duan, Gui-Lan,Zeng, Da-Li,Qian, Qian,Cheng, Wang-Da. 2008

[17]Recent progress on rice genetics in China. Jiang, Hua,Guo, Long-Biao,Qian, Qian. 2007

[18]A model for photothermal responses of flowering in rice .1. Model description and parameterization. Yin, XY,Kropff, MJ,Horie, T,Nakagawa, H,Centeno, HGS,Zhu, DF,Goudriaan, J. 1997

[19]Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice. Zhou, Kunneng,Ren, Yulong,Lv, Jia,Wang, Yihua,Liu, Feng,Zhou, Feng,Zhao, Shaolu,Chen, Saihua,Peng, Cheng,Jiang, Ling,Wan, Jianmin,Zhang, Xin,Guo, Xiuping,Cheng, Zhijun,Wang, Jiulin,Wu, Fuqing,Wan, Jianmin.

[20]A receptor-like protein RMC is involved in regulation of iron acquisition in rice. Yang, An,Zhang, Wen-Hao,Li, Yansu,Xu, Yunyun,Zhang, Wen-Hao.

作者其他论文 更多>>

微信小程序