A Novel Soybean Dirigent Gene GmDIR22 Contributes to Promotion of Lignan Biosynthesis and Enhances Resistance to Phytophthora sojae

文献类型： 外文期刊

第一作者： Li, Ninghui

作者： Li, Ninghui;Zhao, Ming;Liu, Tengfei;Dong, Lidong;Cheng, Qun;Wang, Le;Chen, Xi;Zhang, Chuanzhong;Xu, Pengfei;Zhang, Shuzhen;Li, Ninghui;Wu, Junjiang;Lu, Wencheng

作者机构：

关键词： Glycine max;dirigent protein;lignan;Phytophthora sojae;gene expression

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Phytophthora root and stem rot caused by the oomycete pathogen Phytophthora sojae is a destructive disease of soybean worldwide. Plant dirigent proteins (DIR) are proposed to have roles in biosynthesis of either lignan or lignin-like molecules, and are important for defense responses, secondary metabolism, and pathogen resistance. In the present work, a novel DIR gene expressed sequence tag is identified as up-regulated in the highly resistant soybean cultivar 'Suinong 10' inoculated with P. sojae. The full length cDNA is isolated using rapid amplification of cDNA ends, and designated GmDIR22 (GenBank accession no. HQ_993047). The full length GmDIR22 is 789 bp and contains a 567 bp open reading frame encoding a polypeptide of 188 amino acids. The sequence analysis indicated that GmDIR22 contains a conserved dirigent domain at amino acid residues 43-187. The quantitative real-time reverse transcription PCR demonstrated that soybean GmDIR22 mRNA is expressed most highly in stems, followed by roots and leaves. The treatments with stresses demonstrated that GmDIR22 is significantly induced by P. sojae and gibberellic acid (GA(3)), and also responds to salicylic acid, methyl jasmonic acid, and abscisic acid. The GmDIR22 is targeted to the cytomembrane when transiently expressed in Arabidopsis protoplasts. Moreover, The GmDIR22 recombinant protein purified from Escherichia coli could effectively direct E-coniferyl alcohol coupling into lignan (C)-pinoresinol. Accordingly, the overexpression of GmDIR22 in transgenic soybean increased total lignan accumulation. Moreover, the lignan extracts from GmDIR22 transgenic plants effectively inhibits P. sojae hyphal growth. Furthermore, the transgenic overexpression of GmDIR22 in the susceptible soybean cultivar 'Dongnong 50' enhances its resistance to P. sojae. Collectively, these data suggested that the primary role of GmDIR22 is probably involved in the regulation of lignan biosynthesis, and which contributes to resistance to P. sojae.

分类号：

  • 相关文献

[1]Overexpression of GmERF5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean. Dong, Lidong,Cheng, Yingxin,Cheng, Qun,Li, Wenbin,Fan, Sujie,Jiang, Liangyu,Xu, Pengfei,Zhang, Shuzhen,Wu, Junjiang,Wu, Junjiang,Kong, Fanjiang,Xu, Zhaolong,Zhang, Dayong.

[2]Differentially Expressed Genes of Soybean During Infection by Phytophthora sojae. Xu Peng-fei,Li Wen-bin,Fan Su-jie,Li Ning-hui,Wang Xin,Jiang Liang-yu,Zhang Shu-zhen,Wu Jun-jiang,Wei Lai,Xue, Allen,Chen Wei-yuan,Lv Hui-ying,Lin Shi-feng. 2012

[3]GmWRKY31 and GmHDL56 Enhances Resistance to Phytophthora sojae by Regulating Defense-Related Gene Expression in Soybean. Fan, Sujie,Dong, Lidong,Han, Dan,Jiang, Liangyu,Cheng, Qun,Li, Rongpeng,Meng, Fanshan,Zhang, Shuzhen,Xu, Pengfei,Fan, Sujie,Jiang, Liangyu,Zhang, Feng,Wu, Junjiang,Lu, Wencheng. 2017

[4]A Novel Soybean ERF Transcription Factor, GmERF113, Increases Resistance to Phytophthora sojae Infection in Soybean. Zhao, Yuanling,Chang, Xin,Qi, Dongyue,Dong, Lidong,Fan, Sujie,Jiang, Liangyu,Cheng, Qun,Chen, Xi,Han, Dan,Xu, Pengfei,Zhang, Shuzhen,Zhao, Yuanling,Wang, Guangjin. 2017

[5]Molecular analysis of the annexin gene family in soybean. Wei, X. K.,Liao, W. X.,Zhang, H.,Liang, S. C.,Peng, H.,Huang, L. H.,Peng, H..

[6]Overexpression of cotton (Gossypium hirsutum) dirigent1 gene enhances lignification that blocks the spread of Verticillium dahliae. Haiyan Shi,Zhihao Liu,Li Zhu,Chaojun Zhang,Yun Chen,Ying Zhou,Fuguang Li,Xuebao Li. 2012

[7]Chemical constituents from Orobanche cernua Loefling. Qu, Zheng-yi,Zhang, Yu-wei,Yao, Chun-lin,Jin, Yin-ping,Zheng, Pei-he,Sun, Cheng-he,Liu, Jun-xia,Wang, Yu-shuai,Wang, Ying-ping.

[8]Phenolic compounds from the roots of valeriana officinalis var. latifolia. Wang, Peng-Cheng,Ran, Xin-Hui,Luo, Huai-Rong,Liu, Yu-Qing,Zhou, Jun,Ma, Qing-Yun,Zhao, You-Xing.

[9]Analysis of simple sequence repeats markers derived from Phytophthora sojae expressed sequencetags. Zhu, ZD,Huo, YL,Wang, XM,Huang, JB,Wu, XF. 2004

[10]Phenotypic evaluation and genetic dissection of resistance to Phytophthora sojae in the Chinese soybean mini core collection. Huang, Jing,Guo, Na,Sun, Jutao,Hu, Guanjun,Zhang, Haipeng,Zhang, Xing,Zhao, Jinming,Xing, Han,Li, Yinghui,Li, Yanfei,Qiu, Lijuan. 2016

[11]Fine Mapping and Identification of a Novel Phytophthora Root Rot Resistance Locus RpsZS18 on Chromosome 2 in Soybean. Zhong, Chao,Sun, Suli,Duan, Canxing,Zhu, Zhendong,Yao, Liangliang,Ding, Junjie. 2018

[12]Detached-petiole inoculation method to evaluate Phytophthora root rot resistance in soybean plants. Li, Yinping,Sun, Suli,Zhong, Chao,Zhu, Zhendong.

[13]Isolation and characterization of Bacillus altitudinis JSCX-1 as a new potential biocontrol agent against Phytophthora sojae in soybean [Glycine max (L.) Merr.]. Lu, Xiaoxue,Zhou, Dongmei,Chen, Xi,Zhang, Jinfeng,Huang, Huiwen,Wei, Lihui,Lu, Xiaoxue,Zhou, Dongmei,Chen, Xi,Zhang, Jinfeng,Huang, Huiwen,Wei, Lihui.

[14]Genetic analysis of Phytophthora sojae populations in Fujian, China. Wu, M.,Li, B.,Liu, P.,Weng, Q.,Chen, Q.,Wu, M.,Zhan, J.,Chen, Q..

[15]Isolation and characterization of a pathogenesis-related protein 10 gene (GmPR10) with induced expression in soybean (Glycine max) during infection with Phytophthora sojae. Xu, Pengfei,Jiang, Liangyu,Li, Wenbin,Fan, Sujie,Zhang, Shuzhen,Wu, Junjiang,Wu, Junjiang.

[16]The histone acetyltransferase PsGcn5 mediates oxidative stress responses and is required for full virulence of Phytophthora sojae. Zhao, Wei,Wang, Tao,Liu, Shusen,Chen, Qingqing,Qi, Rende,Zhao, Wei,Wang, Tao,Qi, Rende.

[17]Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos. Li, Ai Qin,Zhao, Chuan Zhi,Wang, Xing Jun,Liu, Zhan Ji,Song, Guo Qi,Yin, Juan,Li, Chang Sheng,Xia, Han,Bi, Yu Ping,Zhang, Li Feng. 2010

[18]Global Analysis of WRKY Genes and Their Response to Dehydration and Salt Stress in Soybean. Song, Hui,Wang, Pengfei,Hou, Lei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Li, Pengcheng,Zhang, Ye,Bian, Xiaotong,Wang, Xingjun. 2016

[19]Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae. Zhang, H. T.,Bi, Y. P.,Liu, Z. J.,Shan, L.. 2009

[20]A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.). Liu, Wei,Han, Xiangdong,Zhan, Ge,Zhao, Zhenfang,Wu, Cunxiang,Han, Xiangdong,Zhan, Ge,Zhao, Zhenfang,Feng, Yongjun. 2015

作者其他论文 更多>>

微信小程序