Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species

文献类型： 外文期刊

第一作者： Jin, Shumei

作者： Jin, Shumei;Wang, Ji;Sun, Dan;Liu, Shengkui;Wang, Xinwang;Genovesi, A. D.;Li, Guoliang

作者机构：

关键词： Callus;Differentiation;In vitro regeneration;Leaf explants;Lilium pumilum;Lily

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN： 1054-5476

年卷期： 2014 年 50 卷 1 期

页码：

收录情况： SCI

摘要： Alternative methods of in vitro cloning that involve both adventitious (direct) and callus intermediate (indirect) pathways were investigated for the endangered species Lilium pumilum. Plantlet regeneration was obtained from leaf explants, cultured on Murashige and Skoog (MS) basal medium supplemented with various combinations of auxins and cytokinins at different concentrations. About 30% of the explants directly formed adventitious shoots on MS medium containing 8.88 mu M 6-benzyladenine (BA) and 2.69 mu M alpha-naphthaleneacetic acid (NAA). For production of regenerable callus, callus formation followed by shoot induction was best when explants were initially cultured on MS medium supplemented with 9.05 mu M 2,4-dichlorophenoxyacetic acid (2,4-D). Regenerable calli were yellow or purple and readily regenerated shoots when subcultured onto MS medium containing 2.22 mu M BA and 1.61 mu M NAA. About 78% of the calli were able to produce adventitious shoots. Shoots were rooted on half-strength MS medium supplemented with 1.34 mu M NAA and were successfully acclimatized to greenhouse conditions. This report describes an efficient method for the in vitro multiplication of whole plants from leaf explants of the endangered species L. pumilum.

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